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PMID: 2119503 Published · ppublish English Journal Article

Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 18 ·1990-09-00 ·Pages 7240-4

Brown P, Liberski PP, Wolff A, Gajdusek DC

Abstract

Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.

MeSH Terms
Animals Brain/microbiology,pathology Cricetinae Electrophoresis, Polyacrylamide Gel Hydrolases Mesocricetus Molecular Weight Prions/isolation & purification Scrapie/microbiology,pathology,transmission
Chemicals
Prions Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brown P
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Liberski P P
Wolff A
Gajdusek D C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.18.7240
Published
1990-09-00
Pages
7240-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54719
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.18.7240 PMC Full Text View journal details
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