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PMID: 2118143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

c-fos expression precedes osteogenic differentiation of cartilage cells in vitro.

The Journal of cell biology ·Vol. 111 ·No. 3 ·1990-09-00 ·Pages 1313-23

Closs EI, Murray AB, Schmidt J, Schön A, Erfle V, Strauss PG

Abstract

We have investigated the temporal pattern of expression of c-fos in cartilage cells in mouse mandibular condyles. During in vitro cultivation, the progenitor cells in this organ differentiate to osteoblasts, and hypertrophic chondrocytes start to show features indicative of osteogenic differentiation. Prior to these processes we observed two distinct patterns of c-fos expression. High, transient c-fos expression was found in the entire tissue within 30 min of culture. This type of c-fos expression appeared to result from mechanical forces applied during dissection. The second type of c-fos expression appeared in individual cells in the zone of hypertrophic chondrocytes. A varying number of formerly quiescent chondrocytes expressed high levels of c-fos mRNA after between 30 min and 10 d in culture, with a peak in the number of cells between days 1 and 3. c-fos expression in these cartilage cells was followed by DNA replication and expression of genes typifying osteoblastic differentiation. After 7 d in culture, groups of cells with the typical ultrastructural features of osteoblasts, and surrounded by an osteoid-like matrix, were observed in single chondrocyte-type lacunae, suggesting division of chondrocytes and differentiation to osteoblasts. The data suggest that c-fos may play a crucial role in the perturbation of determined pathways of skeletoblast differentiation and in the regulation of endochondral bone formation.

MeSH Terms
Animals Autoradiography Cartilage/cytology Cell Differentiation/genetics Cell Division Collagen/genetics Gene Expression Regulation In Vitro Techniques Mice Nucleic Acid Hybridization Osteogenesis/genetics Proto-Oncogene Proteins/biosynthesis,genetics Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc RNA, Messenger/analysis Time Factors
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc RNA, Messenger Collagen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Closs E I
Gesellschaft für Strahlen- und Umweltforschung, Abteilung für Molekulare Zellpathologie, Federal Republic of Germany.
Murray A B
Schmidt J
Schön A
Erfle V
Strauss P G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.3.1313
Published
1990-09-00
Pages
1313-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116259
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.3.1313 PMC Full Text View journal details
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