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PMID: 2110138 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of the hemA region of the Bacillus subtilis chromosome.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2250-8

Petricek M, Rutberg L, Schröder I, Hederstedt L

Abstract

A 3.8-kilobase DNA fragment from Bacillus subtilis containing the hemA gene has been cloned and sequenced. Four open reading frames were identified. The first is hemA, encoding a protein of 50.8 kilodaltons. The primary defect of a B. subtilis 5-aminolevulinic acid-requiring mutant was identified as a cysteine-to-tyrosine substitution in the HemA protein. The predicted amino acid sequence of the B. subtilis HemA protein showed 34% identity with the Escherichia coli HemA protein, which is known to code for the NAD(P)H:glutamyl-tRNA reductase of the C5 pathway for 5-aminolevulinic acid synthesis. The B. subtilis HemA protein also complements the defect of an E. coli hemA mutant. The second open reading frame in the cloned fragment, called ORF2, codes for a protein of about 30 kilodaltons with unknown function. It is not the proposed hemB gene product porphobilinogen synthase. The third open reading frame is hemC, coding for porphobilinogen deaminase. The fourth open reading frame extends past the sequenced fragment and may be identical to hemD, coding for uroporphyrinogen III cosynthase. Analysis of deletion mutants of the hemA region suggests that (at least) hemA, ORF2, and hemC may be part of an operon.

MeSH Terms
Aldehyde Oxidoreductases/genetics Amino Acid Sequence Bacillus subtilis/genetics,growth & development Bacterial Proteins/genetics Base Sequence Chromosome Deletion Chromosomes, Bacterial Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Heme/biosynthesis Molecular Sequence Data Mutation Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Heme Aldehyde Oxidoreductases glutamyl tRNA reductase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Petricek M
Department of Microbiology, University of Lund, Sweden.
Rutberg L
Schröder I
Hederstedt L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2250-2258.1990
Published
1990-05-00
Pages
2250-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208856
Subset
IM
Databases
GENBANK
M57676
External Links
PubMed source DOI: 10.1128/jb.172.5.2250-2258.1990 PMC Full Text View journal details
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