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PMID: 3276659 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of the Escherichia coli K-12 hemB gene.

Journal of bacteriology ·Vol. 170 ·No. 2 ·1988-02-00 ·Pages 1021-5

Li JM, Umanoff H, Proenca R, Russell CS, Cosloy SD

Abstract

An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.

MeSH Terms
Alleles Chromosome Mapping Cloning, Molecular Escherichia coli/enzymology,genetics Genes, Bacterial Heme/biosynthesis,genetics Hydroxymethylbilane Synthase/biosynthesis,genetics Mutation Plasmids Porphobilinogen Synthase/biosynthesis,genetics
Chemicals
Heme Hydroxymethylbilane Synthase Porphobilinogen Synthase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li J M
Department of Biochemistry, City College, City University of New York, New York 10031.
Umanoff H
Proenca R
Russell C S
Cosloy S D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.2.1021-1025.1988
Published
1988-02-00
Pages
1021-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210762
Subset
IM
Grants
NCRR NIH HHS · RR03060 · United States
NCRR NIH HHS · RR08168 · United States
External Links
PubMed source DOI: 10.1128/jb.170.2.1021-1025.1988 PMC Full Text View journal details
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