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PMID: 2109304 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

An anticodon change switches the identity of E. coli tRNA(mMet) from methionine to threonine.

Nucleic acids research ·Vol. 18 ·No. 2 ·1990-01-25 ·Pages 285-9

Schulman LH, Pelka H

Abstract

Recent evidence indicates that the anticodon may often play a crucial role in selection of tRNAs by aminoacyl-tRNA synthetases. In order to quantitate the contribution of the anticodon to discrimination between cognate and noncognate tRNAs by E. coli threonyl-tRNA synthetase, derivatives of the E. coli elongator methionine tRNA (tRNA(mMet)) containing wild type and threonine anticodons have been synthesized in vitro and assayed for threonine acceptor activity. Substitution of the threonine anticodon GGU for the methionine anticodon CAU increased the threonine acceptor activity of tRNA(mMet) by four orders of magnitude while reducing methionine acceptor activity by an even greater amount. These results indicate that the anticodon is the major element which determines the identity of both threonine and methionine tRNAs.

MeSH Terms
Anticodon/genetics Base Sequence Escherichia coli/enzymology,genetics Genes, Bacterial Genes, Synthetic Kinetics Methionine Methionine-tRNA Ligase/isolation & purification,metabolism Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics Threonine Threonine-tRNA Ligase/isolation & purification,metabolism Transcription, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl tRNA(m)(Met), methionine- Threonine RNA, Transfer Methionine Methionine-tRNA Ligase Threonine-tRNA Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schulman L H
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Pelka H
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.2.285
Published
1990-01-25
Pages
285-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330265
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.2.285 PMC Full Text View journal details
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