Factors determining the specificity of the tRNA aminoacylation reaction. Non-absolute specificity of tRNA-aminoacyl-tRNA synthetase recognition and particular importance of the maximal velocity.

Amino Acyl-tRNA Synthetases/antagonists & inhibitors,metabolism Base Sequence Binding Sites Binding, Competitive Centrifugation, Density Gradient Electrophoresis, Disc Escherichia coli/enzymology Kinetics Protein Binding RNA, Transfer/metabolism Saccharomyces cerevisiae/enzymology Structure-Activity Relationship Sucrose Transfer RNA Aminoacylation Valine

Sucrose RNA, Transfer Amino Acyl-tRNA Synthetases Valine