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PMID: 2108969 Published · ppublish English Journal Article

Cytoskeletal control of centrioles movement during the establishment of polarity in Madin-Darby canine kidney cells.

The Journal of cell biology ·Vol. 110 ·No. 4 ·1990-04-00 ·Pages 1123-35

Buendia B, Bré MH, Griffiths G, Karsenti E

Abstract

The two centrioles that are localized close to each other and to the nucleus in single Madin-Darby Canine kidney cells (MDCK) move apart by distances as large as 13 microns after the establishment of extensive cellular junctions. Microfilaments, and possibly microtubules appear to be responsible for this separation. In fully polarized cells, the centrioles are localized just beneath the apical membrane. After disruption of intercellular junctions in low calcium medium, the centrioles move back towards the cell center. This process requires intact microtubules but happens even in the absence of microfilaments. These results indicate that the position of centrioles is determined by opposing forces produced by microtubules and microfilaments and suggest that the balance between these forces is modulated by the assembly of cellular junctions. Centriole separation appears to be an early event in the process that precedes their final positioning in the apical-most region of the polarized cell.

MeSH Terms
Actin Cytoskeleton/ultrastructure Animals Calcium/pharmacology Cell Line Centrioles/drug effects,physiology,ultrastructure Culture Media Cytochalasin D/pharmacology Cytoskeleton/drug effects,physiology,ultrastructure Dogs Egtazic Acid/pharmacology Fluorescent Antibody Technique Kidney Kinetics Microtubules/ultrastructure Models, Structural Nocodazole/pharmacology
Chemicals
Culture Media Cytochalasin D Egtazic Acid Nocodazole Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buendia B
European Molecular Biology Laboratory, Heidelberg, FRG.
Bré M H
Griffiths G
Karsenti E
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44 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.4.1123
Published
1990-04-00
Pages
1123-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116076
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.110.4.1123 PMC Full Text View journal details
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