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PMID: 2105453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway.

Molecular and cellular biology ·Vol. 10 ·No. 2 ·1990-02-00 ·Pages 510-7

Cole GM, Stone DE, Reed SI

Abstract

The Saccharomyces cerevisiae GPA1, STE4, and STE18 genes encode products homologous to mammalian G-protein alpha, beta, and gamma subunits, respectively. All three genes function in the transduction of the signal generated by mating pheromone in haploid cells. To characterize more completely the role of these genes in mating, we have conditionally overexpressed GPA1, STE4, and STE18, using the galactose-inducible GAL1 promoter. Overexpression of STE4 alone, or STE4 together with STE18, generated a response in haploid cells suggestive of pheromone signal transduction: arrest in G1 of the cell cycle, formation of cellular projections, and induction of the pheromone-inducible transcript FUS1 25- to 70-fold. High-level STE18 expression alone had none of these effects, nor did overexpression of STE4 in a MATa/alpha diploid. However, STE18 was essential for the response, since overexpression of STE4 was unable to activate a response in a ste18 null strain. GPA1 hyperexpression suppressed the phenotype of STE4 overexpression. In addition, cells that overexpressed GPA1 were more resistant to pheromone and recovered more quickly from pheromone than did wild-type cells, which suggests that GPA1 may function in an adaptation response to pheromone.

MeSH Terms
Cloning, Molecular GTP-Binding Proteins/genetics,metabolism Galactose/pharmacology Gene Expression Genes, Fungal/drug effects Kinetics Mating Factor Peptides/pharmacology Pheromones/pharmacology Plasmids Saccharomyces cerevisiae/drug effects,genetics,growth & development Signal Transduction
Chemicals
Peptides Pheromones Mating Factor GTP-Binding Proteins Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cole G M
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Stone D E
Reed S I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.2.510-517.1990
Published
1990-02-00
Pages
510-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360826
Subset
IM
Grants
NIGMS NIH HHS · GM49429 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.2.510-517.1990 PMC Full Text View journal details
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