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PMID: 2548076 Published · ppublish English Journal Article

GPA1Val-50 mutation in the mating-factor signaling pathway in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 6 ·1989-06-00 ·Pages 2289-97

Miyajima I, Arai K, Matsumoto K

Abstract

The GPA1 gene of Saccharomyces cerevisiae encodes a protein that is highly homologous to the alpha subunit of mammalian hetrotrimeric G proteins and is essential for haploid cell growth. A mutation of the GPA1 protein, GPA1Val-50, in which Gly-50 was replaced by valine, could complement the growth defect of a GPA1 disruption, gpal::HIS3. However, cells with gpa1::HIS3 expressing the GPA1Val-50 protein were supersensitive to alpha-factor in a short-term incubation but resumed growth after long-term incubation even after exposure to high concentrations of alpha-factor. The former phenotype associated with GPA1Val-50 is recessive, and the latter phenotype is dominant to GPA1+. The supersensitivity of GPA1Val-50 to alpha-factor was dependent on STE2 and STE4, which demonstrates that this GPA1Val-50-produced phenotype requires the mating-factor receptor and the beta subunit of the G protein. The double mutant of sst2-1 GPA1Val-50 recovered from division arrest, which suggested that SST2 is not required for recovery of the GPA1Val-50 mutant.

MeSH Terms
Amino Acid Sequence Cell Division GTP-Binding Proteins/genetics,physiology Genes, Fungal Genetic Complementation Test Glycine/physiology Mating Factor Molecular Sequence Data Mutation Peptides/physiology Phenotype Plasmids Receptors, Cell Surface/physiology Receptors, Mating Factor Receptors, Peptide Saccharomyces cerevisiae/cytology,genetics,growth & development Signal Transduction Transcription Factors Valine/physiology beta-Galactosidase/genetics
Chemicals
Peptides Receptors, Cell Surface Receptors, Mating Factor Receptors, Peptide Transcription Factors Mating Factor beta-Galactosidase GTP-Binding Proteins Valine Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miyajima I
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.
Arai K
Matsumoto K
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.6.2289-2297.1989
Published
1989-06-00
Pages
2289-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362301
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.6.2289-2297.1989 PMC Full Text View journal details
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