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PMID: 20973995 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes.

BMC plant biology ·Vol. 10 ·2010-10-25 ·Pages 230

Huang XS, Liu JH, Chen XJ

Abstract

Drought is one of the major abiotic stresses affecting plant growth, development and crop productivity. ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes. In this study, a gene coding for ABF (PtrABF) was isolated from Poncirus trifoliata (L.) Raf. PtrABF had a complete open reading frame of 1347 bp, encoding a 448 amino acid peptide, and shared high sequence identities with ABFs from other plants. PtrABF was subcellularly targeted to the nucleus, exhibited transactivation activity in yeast cell and could bind to ABRE, supporting its role as a transcription factor. Expression levels of PtrABF were induced by treatments with dehydration, low temperature and ABA. Ectopic expression of PtrABF under the control of a CaMV 35S promoter in transgenic tobacco plants enhanced tolerance to both dehydration and drought. Under dehydration and drought conditions, the transgenic plants accumulated lower levels of reactive oxygen species compared with wild type, accompanied by higher activities and expression levels of three antioxidant enzymes. In addition, steady-state mRNA levels of nine stress-responsive genes coding for either functional or regulatory proteins were induced to higher levels in the transgenic lines with or without drought stress. PtrABF is a bZIP transcription factor and functions in positive modulation of drought stress tolerance. It may be an important candidate gene for molecular breeding of drought-tolerant plants.

MeSH Terms
Abscisic Acid/pharmacology Adaptation, Physiological/drug effects Amino Acid Sequence Basic-Leucine Zipper Transcription Factors/classification,genetics Catalase/metabolism Cell Nucleus/metabolism Gene Expression Regulation, Plant/drug effects Green Fluorescent Proteins/genetics,metabolism Molecular Sequence Data Peroxidase/metabolism Phylogeny Plant Growth Regulators/pharmacology Plant Proteins/classification,genetics Plants, Genetically Modified Poncirus/genetics Reactive Oxygen Species/metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Stress, Physiological/drug effects Superoxide Dismutase/metabolism Temperature Tobacco/genetics,metabolism Water/metabolism,pharmacology
Chemicals
Basic-Leucine Zipper Transcription Factors Plant Growth Regulators Plant Proteins Reactive Oxygen Species Water Green Fluorescent Proteins Abscisic Acid Catalase Peroxidase Superoxide Dismutase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Huang Xiao-San
National Key Laboratory of Crop Genetic Improvement, Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070 China.
Liu Ji-Hong
Chen Xue-Jun
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Article Info
Journal
BMC plant biology
Abbr.
BMC Plant Biol
ISSN
1471-2229
DOI
10.1186/1471-2229-10-230
Published
2010-10-25
Epub
2010-00-25
Pages
230
Language
English
Region
England
NLM ID
100967807
PMCID
PMC3017851
Subset
IM
Databases
GENBANK
HM171703
External Links
PubMed source DOI: 10.1186/1471-2229-10-230 PMC Full Text View journal details
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