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PMID: 20846372 Published · epublish English Journal Article Research Support, N.I.H., Extramural

HIV-1 is budded from CD4+ T lymphocytes independently of exosomes.

Virology journal ·Vol. 7 ·2010-09-16 ·Pages 234

Park IW, He JJ

Abstract

The convergence of HIV-1 budding and exosome biogenesis at late endosomal compartments called multivesicular bodies has fueled the debate on whether HIV-1 is budded from its target cells and transmitted in the form of exosomes. The point of contention appears to primarily derive from the types of target cells in question and lack of a well-defined protocol to separate exosomes from HIV-1. In this study, we adapted and established a simplified protocol to define the relationship between HIV-1 production and exosome biogenesis. Importantly, we took advantage of the newly established protocol to unequivocally show that HIV-1 was produced from CD4+ T lymphocytes Jurkat cells independently of exosomes. Thus, this study not only presents a simplified way to obtain highly purified HIV-1 virions for identification of host proteins packaged into virions, but also provides a technical platform that can be employed to define the relationship between exosome biogenesis and budding of HIV-1 or other viruses and its contributions to viral pathogenesis.

MeSH Terms
CD4-Positive T-Lymphocytes/virology Cell Line Exosomes/ultrastructure,virology HIV-1/physiology,ultrastructure Humans Microscopy, Electron, Transmission Virion/ultrastructure Virus Release
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Park In-Woo
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
He Johnny J
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Article Info
Journal
Virology journal
Abbr.
Virol J
ISSN
1743-422X
DOI
10.1186/1743-422X-7-234
Published
2010-09-16
Epub
2010-00-16
Pages
234
Language
English
Region
England
NLM ID
101231645
PMCID
PMC2945958
Subset
IM
Grants
NIMH NIH HHS · R01MH065158 · United States
NIDA NIH HHS · R21DA029428 · United States
External Links
PubMed source DOI: 10.1186/1743-422X-7-234 PMC Full Text View journal details
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