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PMID: 20740026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unveiling an abundant core microbiota in the human adult colon by a phylogroup-independent searching approach.

The ISME journal ·Vol. 5 ·No. 3 ·2011-03-00 ·Pages 519-31

Sekelja M, Berget I, Næs T, Rudi K

Abstract

The potential presence of widespread and stable bacterial core phylogroups in the human colon has promoted considerable attention. Despite major efforts, no such phylogroups have yet been identified. Therefore, using a novel phylogroup- and tree-independent approach, we present a reanalysis of 1,114,722 V2 region and 71,550 near full-length 16S rRNA sequences from a total of 210 human beings, with widespread geographic origin, ethnic background and diet, in addition to a wide range of other mammals. We found two highly prevalent core phylogroups (cores 1 and 2), belonging to the clostridial family Lachnospiraceae. These core phylogroups showed a log-normal distribution among human individuals, while non-core phylogroups showed more skewed distributions towards individuals with low levels compared with the log-normal distribution. Molecular clock analyses suggest that core 2 co-evolved with the radiation of vertebrates, while core 1 co-evolved with the mammals. Taken together, the stability, prevalence and potential functionality support the fact that the identified core phylogroups are pivotal in maintaining gut homeostasis and health.

MeSH Terms
Animals Bacteria/classification,genetics Base Sequence Colon/microbiology Gram-Positive Bacteria/classification,genetics Humans Metagenome Phylogeny RNA, Ribosomal, 16S/genetics
Chemicals
RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sekelja Monika
Nofima, The Norwegian Institute of Food, Fisheries and Aquaculture Research, Aas, Norway. monika.sekelja@nofima.no
Berget Ingunn
Næs Tormod
Rudi Knut
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Article Info
Journal
The ISME journal
Abbr.
ISME J
ISSN
1751-7370
DOI
10.1038/ismej.2010.129
Email
monika.sekelja@nofima.no
Published
2011-03-00
Epub
2010-00-26
Pages
519-31
Language
English
Region
England
NLM ID
101301086
PMCID
PMC3105728
Subset
IM
External Links
PubMed source DOI: 10.1038/ismej.2010.129 PMC Full Text View journal details
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