Complete loss of heparin-releasable triacylglycerol lipase activity after collagenase treatment of the rat liver.

The Biochemical journal ·Vol. 172 ·No. 1 ·1978-04-15 ·Pages 177-9

Thomas J, Debeer LJ, Mannaerts GP

Abstract

Lipolytic activity at pH 8.5-9.5 was lowered by approx. 80% in homogenates from livers perfused with collagenase or heparin. No heparin-releasable lipase activity was detected in hepatocytes isolated by collagenase treatment. It is concluded that crude collagenase completely inactivates the plasma-membrane-bound heparin-releasable lipase.

MeSH Terms

Animals Heparin/pharmacology In Vitro Techniques Lipase/antagonists & inhibitors Liver/drug effects,enzymology Male Microbial Collagenase/pharmacology Rats

Chemicals

Heparin Lipase Microbial Collagenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Thomas J

Debeer L J

Mannaerts G P

References (13)

13 references, click to expand

Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Selective measurement of two different triglyceride lipase activities in rat postheparin plasma.
J Lipid Res. 1973 May;14(3):286-95 PMID: 9704072
Uptake and degradation of cholesterol ester-labelled rat plasma lipoproteins in purified rat hepatocytes and nonparenchymal liver cells.
Biochim Biophys Acta. 1977 Apr 26;487(1):122-36 PMID: 192303
Effect of sulfonylureas on triglyceride metabolism in the rat liver: possible role of the lysosomes in hepatic lipolysis.
J Clin Invest. 1977 Feb;59(2):185-92 PMID: 13085
Effects of anti-microtubular agents and cycloheximide on the metabolism of chylomicron cholesteryl esters by hepatocyte suspensions.
Biochem J. 1977 Feb 15;162(2):367-77 PMID: 192220
Identification of an adipose tissue-like lipoprotein lipase in perfusates of chicken liver.
J Lipid Res. 1977 Nov;18(6):768-73 PMID: 925521
Gluconeogenesis in the perfused rat liver.
Biochem J. 1966 Nov;101(2):284-92 PMID: 5966267
Rat high density lipoprotein subfraction (HDL3) uptake and catabolism by isolated rat liver parenchymal cells.
J Biol Chem. 1976 Aug 25;251(16):4914-21 PMID: 182684
Metabolic effects of hypoglycemic sulfonylureas. 3. Effect of chlorpropamide on energy metabolism and ketogenesis in the isolated perfused rat liver.
Biochem Pharmacol. 1974 Jan 1;23(1):75-87 PMID: 4811060
Characterization, subcellular localization, and partial purification of a heparin-released triglyceride lipase from rat liver.
J Biol Chem. 1973 Mar 25;248(6):1992-9 PMID: 4690590
Uptake of chyle cholesterol esters and intact triglycerides by suspended hepatocytes.
FEBS Lett. 1975 Mar 1;51(1):219-24 PMID: 1123058
Lysosomal lipases of rat liver and kidney.
J Biol Chem. 1968 Jun 10;243(11):2849-54 PMID: 5653176
Comparison of the triglyceride lipase of liver, adipose tissue, and postheparin plasma.
J Lipid Res. 1972 May;13(3):356-63 PMID: 4337156

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1720177

Published

1978-04-15

Pages

177-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1185676

Subset

External Links

PubMed source DOI: 10.1042/bj1720177 PMC Full Text View journal details

Analysis Services

Complete loss of heparin-releasable triacylglycerol lipase activity after collagenase treatment of the rat liver.

Contact

WeChat Official Account