Home LiteratureArticle Details
PMID: 206266 Published · ppublish English Journal Article

Problems encountered in measuring the activity of phosphatidate phosphohydrolase.

The Biochemical journal ·Vol. 171 ·No. 1 ·1978-04-01 ·Pages 263-6

Sturton RG, Brindley DN

Abstract

The measurement of phosphate release from phosphatidate overestimates the microsomal activity of phosphatidate phosphohydrolase from rat liver, since phosphate is also produced via the glycerol phosphate that results from the deacylation of phosphatidate. The determination of phosphate production can be a reliable assay for the soluble phosphatidate phosphohydrolase in rat liver, because the glycerol phosphate formed is not hydrolysed under the conditions used.

MeSH Terms
Animals Glycerophosphates/metabolism In Vitro Techniques Liver/drug effects,enzymology Microsomes, Liver/enzymology Phosphatidic Acids Phosphatidylcholines/pharmacology Phosphoric Monoester Hydrolases/metabolism Rats
Chemicals
Glycerophosphates Phosphatidic Acids Phosphatidylcholines Phosphoric Monoester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sturton R G
Brindley D N
References (22)
22 references, click to expand
  1. The nature of the stimulatory role of the supernatant fraction on triglyceride synthesis by the alpha-Glycerophosphate pathway.
    Lipids. 1967 Jan;2(1):14-20 PMID: 17805715
  2. Metabolism of phospholipids. V. Studies of phosphatidic acid phosphatase.
    Biochim Biophys Acta. 1962 Jan 29;56:479-90 PMID: 13880482
  3. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  4. The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycerolipid synthesis in rat liver.
    Biochem J. 1977 Nov 15;168(2):147-53 PMID: 202260
  5. The effects of acute ethanol feeding and of chronic benfluorex administration on the activities of some enzymes of glycerolipid synthesis in rat liver and adipose tissue.
    Biochem J. 1977 Sep 15;166(3):639-42 PMID: 597246
  6. Stimulation of hepatic phosphatidate phosphohydrolase activity by a single dose of ethanol;.
    Biochem Biophys Res Commun. 1977 Mar 21;75(2):511-8 PMID: 192236
  7. Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions.
    Biochem J. 1977 Jan 15;162(1):25-32 PMID: 192211
  8. Some aspects of the physiological and pharmacological control of the synthesis of triacylglycerols and phospholipids.
    Int J Obes. 1978;2(1):7-16 PMID: 213394
  9. The effects of amphiphilic cationic drugs and inorganic cations on the activity of phosphatidate phosphohydrolase.
    Biochem J. 1977 Sep 1;165(3):447-54 PMID: 200224
  10. Phosphatidate phosphohydrolase and the regulation of glycerolipid biosynthesis.
    Biochem Soc Trans. 1977;5(1):37-40 PMID: 196962
  11. The role of phosphatidate phosphohydrolase in glyceride biosynthesis.
    Eur J Biochem. 1967 Dec;3(1):70-7 PMID: 4295052
  12. Mono- and dimethyl phosphatidates from different subtypes of choline and ethanolamine glycerophosphatides.
    Biochim Biophys Acta. 1968 Jan 10;152(1):114-35 PMID: 4297132
  13. Microsomal synthesis of the ether analogs of triacylglycerols. Acyl CoA:alkylacylglycerol and acyl CoA:alk-i-enylacylglycerol acyltransferases in tumors and liver.
    Biochim Biophys Acta. 1974 Jun 26;348(3):397-403 PMID: 4367974
  14. The selective loss of lysophospholipids in some commonly used lipid-extraction procedures.
    Anal Biochem. 1974 Mar;58(1):238-45 PMID: 4825376
  15. Glycerolipid synthesis in rat adipose tissue. II. Properties and distribution of phosphatidate phosphatase.
    J Lipid Res. 1973 Sep;14(5):517-24 PMID: 4354154
  16. The control of lipid biosynthesis in liver particles.
    Ann N Y Acad Sci. 1968 Nov 21;149(2):784-90 PMID: 4306133
  17. Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate.
    J Biol Chem. 1968 Apr 10;243(7):1609-16 PMID: 4296688
  18. Phosphatidate phosphohydrolase and palmitoyl-coenzyme A hydrolase in cardiac subcellular fractions of hyperthyroid rabbits and cardiomyopathic hamsters.
    Biochem J. 1975 Nov;152(2):313-23 PMID: 177001
  19. Drugs affecting the synthesis of glycerides and phospholipids in rat liver. The effects of clofibrate, halofenate, fenfluramine, amphetamine, cinchocaine, chlorpromazine, demethylimipramine, mepyramine and some of their derivatives.
    Biochem J. 1975 Jun;148(3):461-9 PMID: 1200988
  20. Partial purification and properties of microsomal phosphatidate phosphohydrolase from rat liver.
    Biochim Biophys Acta. 1975 Nov 21;409(2):201-11 PMID: 64
  21. Partial purification, properties, and subcellulsr distribution of rat liver phosphatidate phosphatase.
    J Biochem. 1975 Mar;77(3):501-9 PMID: 238959
  22. Phosphatidic acid metabolism in rat liver microsomes.
    Eur J Biochem. 1976 Apr 15;64(1):301-5 PMID: 179810
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1710263
Published
1978-04-01
Pages
263-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1184155
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1710263 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2