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PMID: 20581308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genome-wide distribution of transposed Dissociation elements in maize.

The Plant cell ·Vol. 22 ·No. 6 ·2010-06-00 ·Pages 1667-85

Vollbrecht E, Duvick J, Schares JP, Ahern KR, Deewatthanawong P, Xu L, Conrad LJ, Kikuchi K, Kubinec TA, Hall BD, Weeks R, Unger-Wallace E, Muszynski M, Brendel VP, Brutnell TP

Abstract

The maize (Zea mays) transposable element Dissociation (Ds) was mobilized for large-scale genome mutagenesis and to study its endogenous biology. Starting from a single donor locus on chromosome 10, over 1500 elements were distributed throughout the genome and positioned on the maize physical map. Genetic strategies to enrich for both local and unlinked insertions were used to distribute Ds insertions. Global, regional, and local insertion site trends were examined. We show that Ds transposed to both linked and unlinked sites and displayed a nonuniform distribution on the genetic map around the donor r1-sc:m3 locus. Comparison of Ds and Mutator insertions reveals distinct target preferences, which provide functional complementarity of the two elements for gene tagging in maize. In particular, Ds displays a stronger preference for insertions within exons and introns, whereas Mutator insertions are more enriched in promoters and 5'-untranslated regions. Ds has no strong target site consensus sequence, but we identified properties of the DNA molecule inherent to its local structure that may influence Ds target site selection. We discuss the utility of Ds for forward and reverse genetics in maize and provide evidence that genes within a 2- to 3-centimorgan region flanking Ds insertions will serve as optimal targets for regional mutagenesis.

MeSH Terms
Chromosome Mapping Chromosomes, Plant DNA Transposable Elements DNA, Plant/genetics Genome, Plant Mutagenesis, Insertional Sequence Analysis, DNA Zea mays/genetics
Chemicals
DNA Transposable Elements DNA, Plant
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Vollbrecht Erik
Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA. vollbrec@iastate.edu
Duvick Jon
Schares Justin P
Ahern Kevin R
Deewatthanawong Prasit
Xu Ling
Conrad Liza J
Kikuchi Kazuhiro
Kubinec Tammy A
Hall Bradford D
Weeks Rebecca
Unger-Wallace Erica
Muszynski Michael
Brendel Volker P
Brutnell Thomas P
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.109.073452
Email
vollbrec@iastate.edu
Published
2010-06-00
Epub
2010-00-25
Pages
1667-85
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2910982
Subset
IM
Corrections
CommentIn
PMID: 20695122
External Links
PubMed source DOI: 10.1105/tpc.109.073452 PMC Full Text View journal details
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