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PMID: 2852139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The mutation bronze-mutable 4 derivative 6856 in maize is caused by the insertion of a novel 6.7-kilobase pair transposon in the untranslated leader region of the bronze-1 gene.

Genetics ·Vol. 120 ·No. 3 ·1988-11-00 ·Pages 779-90

Klein AS, Clancy M, Paje-Manalo L, Furtek DB, Hannah LC, Nelson OE

Abstract

The Ds-controlled allele, bz-m4 Derivative 6856 [bz-m4 D6856], is reported to have an altered temporal- and tissue-specific pattern of gene expression. We have cloned this allele and have characterized it at the molecular level. The mutation was caused by the insertion of a complex transposon-like structure 36 base pairs downstream from the Bz mRNA cap site. The insert is 6.7-kbp long. Ds elements, each approximately 2 kbp in length, are at both ends of the insert. The sequence between the Ds elements is a partial duplication of flanking sequences from the 3' end of the Bz gene. These data suggest that Ds initially inserted near the 3' end of the gene and mobilized adjacent sequences as it transposed.

MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics Gene Expression Regulation Glucosyltransferases/genetics Models, Genetic Molecular Sequence Data Mutation Restriction Mapping Zea mays/genetics
Chemicals
DNA Transposable Elements Glucosyltransferases flavonol 3-O-glucosyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Klein A S
Department of Biochemistry, University of New Hampshire, Durham 03824.
Clancy M
Paje-Manalo L
Furtek D B
Hannah L C
Nelson O E
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/120.3.779
Published
1988-11-00
Pages
779-90
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203556
Subset
IM
Databases
GENBANK
X12951, X12969, X12970
External Links
PubMed source DOI: 10.1093/genetics/120.3.779 PMC Full Text View journal details
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