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PMID: 2056283 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The T cell receptor repertoire influences V beta element usage in response to myoglobin.

The Journal of experimental medicine ·Vol. 174 ·No. 1 ·1991-07-01 ·Pages 83-92

Ruberti G, Gaur A, Fathman CG, Livingstone AM

Abstract

T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110-121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) V beta usage in DBA/2 mice. All clones previously tested used the same V beta 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpW Mb protein or peptide 110-121. Only the V beta 8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V beta genes, lacked V beta 8+ peripheral T cells. DBA/2 mice depleted of V beta 8+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2V beta a mice (homozygous for a deletion of multiple V beta gene segments including the V beta 8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V beta a mice had a very restricted TCR V beta repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2V beta a and F23.1-treated DBA/2 mice are discussed.

MeSH Terms
Animals Antigen-Presenting Cells/immunology Cells, Cultured Chromosome Deletion Crosses, Genetic Flow Cytometry Histocompatibility Antigens Class II/immunology Homozygote Kinetics L Cells/immunology Lymph Nodes/immunology Lymphocyte Activation Macromolecular Substances Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Inbred Strains Myoglobin/immunology Receptors, Antigen, T-Cell/genetics,immunology T-Lymphocytes/immunology Transfection
Chemicals
Histocompatibility Antigens Class II Macromolecular Substances Myoglobin Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ruberti G
Department of Medicine, Stanford University School of Medicine, California 94305.
Gaur A
Fathman C G
Livingstone A M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.174.1.83
Published
1991-07-01
Pages
83-92
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118891
Subset
IM
Grants
NIAID NIH HHS · AI-27989 · United States
External Links
PubMed source DOI: 10.1084/jem.174.1.83 PMC Full Text View journal details
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