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PMID: 20549538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of cancer stem cell-like cells from human epithelial ovarian carcinoma cell line.

Cellular and molecular life sciences : CMLS ·Vol. 67 ·No. 22 ·2010-11-00 ·Pages 3915-25

Shi MF, Jiao J, Lu WG, Ye F, Ma D, Dong QG, Xie X

Abstract

Cancer stem cells (CSCs) play an important role in the development, invasion, and drug resistance of carcinoma, but the exact phenotype and characteristics of ovarian CSCs are still disputable. In this study, we identified cancer stem cell-like cells (CSC-LCs) and investigated their characteristics from the ovarian adenocarcinoma cell line 3AO. Our results showed that CSC-LCs were enriched in sphere-forming test and highly expressed CD44(+)CD24⁻. The spheres and CD24⁻ cells possessed strong tumorigenic ability by transplantation into nonobese diabetic/severe combined immunodeficient mice. CD44(+)CD24⁻ cells expressed stem cell markers and differentiated to CD44(+)CD24(+) cells by immunofluorescence assay and fluorescence-activated cell-sorting analysis. In vitro experiments verified that CD44(+)CD24⁻ cells were markedly resistant to carboplatin and paclitaxol. In conclusion, our study identifies the CD44(+)CD24⁻ phenotype, self-renewal, high tumorigenicity, differentiation potential, and drug resistance of ovarian CSC-LCs. Our findings may provide the evidence needed to explore a new strategy in the treatment of ovarian cancer.

MeSH Terms
Adenocarcinoma/drug therapy,immunology,pathology Animals Antineoplastic Agents/therapeutic use CD24 Antigen/immunology Cell Line, Tumor Drug Resistance, Neoplasm Female Humans Hyaluronan Receptors/immunology Mice Mice, SCID Neoplastic Stem Cells/drug effects,immunology,pathology Ovarian Neoplasms/drug therapy,immunology,pathology
Chemicals
Antineoplastic Agents CD24 Antigen Hyaluronan Receptors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Shi M F
Department of Gynecologic Oncology, Women's Hospital, Zhejiang University, Hangzhou, China.
Jiao J
Lu W G
Ye F
Ma D
Dong Q G
Xie X
References (41)
41 references, click to expand
  1. Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 3' kinase/AKT pathways.
    Oncogene. 2005 Nov 14;24(50):7443-54 PMID: 16288291
  2. Lack of expression of c-KIT in ovarian cancers is associated with poor prognosis.
    Int J Cancer. 2000 May 20;89(3):242-50 PMID: 10861500
  3. Cancer stem cells.
    Neoplasma. 2005;52(6):435-40 PMID: 16284686
  4. Coexpression of the c-kit receptor and the stem cell factor in gynecological tumors.
    Cancer Res. 1994 Jun 1;54(11):3049-53 PMID: 7514496
  5. Prospective identification of tumorigenic breast cancer cells.
    Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3983-8 PMID: 12629218
  6. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell.
    Nat Med. 1997 Jul;3(7):730-7 PMID: 9212098
  7. Self-renewal of teratocarcinoma and embryonic stem cells.
    Oncogene. 2004 Sep 20;23(43):7150-60 PMID: 15378075
  8. Epithelial-mesenchymal transitions: twist in development and metastasis.
    Cell. 2004 Aug 6;118(3):277-9 PMID: 15294153
  9. NF-kappaB and epithelial to mesenchymal transition of cancer.
    J Cell Biochem. 2008 Jun 1;104(3):733-44 PMID: 18253935
  10. Brain cancer stem-like cells.
    Eur J Cancer. 2006 Jun;42(9):1237-42 PMID: 16632342
  11. Cancer stem cells--perspectives on current status and future directions: AACR Workshop on cancer stem cells.
    Cancer Res. 2006 Oct 1;66(19):9339-44 PMID: 16990346
  12. The role of cancer stem cells and the side population in epithelial ovarian cancer.
    Histol Histopathol. 2010 Jan;25(1):113-20 PMID: 19924647
  13. Retention of intrinsic stem cell hierarchies in carcinoma-derived cell lines.
    Cancer Res. 2005 Oct 1;65(19):8944-50 PMID: 16204067
  14. Cancer stem cells.
    N Engl J Med. 2006 Sep 21;355(12):1253-61 PMID: 16990388
  15. Cancer stem cells: implications for the progression and treatment of metastatic disease.
    J Cell Mol Med. 2008 Apr;12(2):374-90 PMID: 18182063
  16. Identification of a cancer stem cell in human brain tumors.
    Cancer Res. 2003 Sep 15;63(18):5821-8 PMID: 14522905
  17. Cancer stem cells: models and concepts.
    Annu Rev Med. 2007;58:267-84 PMID: 17002552
  18. Identification and expansion of human colon-cancer-initiating cells.
    Nature. 2007 Jan 4;445(7123):111-5 PMID: 17122771
  19. Cyclic AMP in ovarian cancer cells both inhibits proliferation and increases c-KIT expression.
    Exp Cell Res. 2002 Feb 1;273(1):95-106 PMID: 11795950
  20. Identification and characterization of ovarian cancer-initiating cells from primary human tumors.
    Cancer Res. 2008 Jun 1;68(11):4311-20 PMID: 18519691
  21. Identification of human brain tumour initiating cells.
    Nature. 2004 Nov 18;432(7015):396-401 PMID: 15549107
  22. Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells.
    Biochem Biophys Res Commun. 2006 Dec 29;351(4):820-4 PMID: 17097610
  23. Stem and progenitor-like cells contribute to the aggressive behavior of human epithelial ovarian cancer.
    Cancer Res. 2005 Apr 15;65(8):3025-9 PMID: 15833827
  24. Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression.
    Med Mol Morphol. 2009 Jun;42(2):82-91 PMID: 19536615
  25. Identification and expansion of the tumorigenic lung cancer stem cell population.
    Cell Death Differ. 2008 Mar;15(3):504-14 PMID: 18049477
  26. Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis.
    Carcinogenesis. 2005 Feb;26(2):495-502 PMID: 15513931
  27. Prospective identification of tumorigenic prostate cancer stem cells.
    Cancer Res. 2005 Dec 1;65(23):10946-51 PMID: 16322242
  28. Expression of CD133-1 and CD133-2 in ovarian cancer.
    Int J Gynecol Cancer. 2008 May-Jun;18(3):506-14 PMID: 17868344
  29. A tumorigenic subpopulation with stem cell properties in melanomas.
    Cancer Res. 2005 Oct 15;65(20):9328-37 PMID: 16230395
  30. Platelet-derived growth factor-D overexpression contributes to epithelial-mesenchymal transition of PC3 prostate cancer cells.
    Stem Cells. 2008 Jun;26(6):1425-35 PMID: 18403754
  31. The early detection of ovarian cancer: from traditional methods to proteomics. Can we really do better than serum CA-125?
    Am J Obstet Gynecol. 2008 Sep;199(3):215-23 PMID: 18468571
  32. Epithelial-mesenchymal transitions in tumour progression.
    Nat Rev Cancer. 2002 Jun;2(6):442-54 PMID: 12189386
  33. Recent advances in cancer stem/progenitor cell research: therapeutic implications for overcoming resistance to the most aggressive cancers.
    J Cell Mol Med. 2007 Sep-Oct;11(5):981-1011 PMID: 17979879
  34. Stem cells, cancer, and cancer stem cells.
    Nature. 2001 Nov 1;414(6859):105-11 PMID: 11689955
  35. Detection of tumor stem cell markers in pancreatic carcinoma cell lines.
    Hepatobiliary Pancreat Dis Int. 2007 Feb;6(1):92-7 PMID: 17287174
  36. Prostate cancer stem/progenitor cells: identification, characterization, and implications.
    Mol Carcinog. 2007 Jan;46(1):1-14 PMID: 16921491
  37. Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells.
    Oncogene. 2009 Jan 15;28(2):209-18 PMID: 18836486
  38. Identification of pancreatic cancer stem cells.
    Cancer Res. 2007 Feb 1;67(3):1030-7 PMID: 17283135
  39. CD133: molecule of the moment.
    J Pathol. 2008 Jan;214(1):3-9 PMID: 18067118
  40. Breast cancer stem cells: an overview.
    Eur J Cancer. 2006 Jun;42(9):1219-24 PMID: 16624548
  41. ABC transporters as phenotypic markers and functional regulators of stem cells.
    Stem Cells. 2002;20(1):11-20 PMID: 11796918
Article Info
Journal
Cellular and molecular life sciences : CMLS
Abbr.
Cell Mol Life Sci
ISSN
1420-9071
DOI
10.1007/s00018-010-0420-9
Published
2010-11-00
Epub
2010-00-12
Pages
3915-25
Language
English
Region
Switzerland
NLM ID
9705402
Subset
IM
External Links
PubMed source DOI: 10.1007/s00018-010-0420-9 View journal details
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