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PMID: 2053984 Published · ppublish English Journal Article

Escherichia coli kgtP encodes an alpha-ketoglutarate transporter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3802-6

Seol W, Shatkin AJ

Abstract

The witA gene located between pss and rrnG on the Escherichia coli chromosome encodes a 432-amino acid protein. It is homologous to a human hepatoma glucose transporter and to E. coli membrane proteins that transport citrate (CitA), arabinose (AraE), and xylose (XylE), and, like these carrier proteins, WitA also contains 12 highly hydrophobic putative membrane-spanning regions. Gene disruption mutants constructed in two E. coli strains grew slowly or not at all, depending on genetic background, in M9 minimal medium containing alpha-ketoglutarate. Growth on alpha-ketoglutarate and uptake of alpha-[14C]ketoglutarate were restored by transformation with plasmids containing witA. These complementation studies indicate that WitA is an alpha-ketoglutarate transporter and should be renamed kgtP(alpha-ketoglutarate permease).

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Carrier Proteins/genetics Cloning, Molecular DNA Mutational Analysis DNA, Bacterial/genetics Dicarboxylic Acid Transporters Escherichia coli/genetics,metabolism Escherichia coli Proteins Genes, Bacterial Genetic Complementation Test Ketoglutaric Acids/metabolism Membrane Transport Proteins/genetics Molecular Sequence Data Restriction Mapping
Chemicals
Carrier Proteins DNA, Bacterial Dicarboxylic Acid Transporters Escherichia coli Proteins Ketoglutaric Acids Membrane Transport Proteins KgtP protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seol W
Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08854-5638.
Shatkin A J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3802
Published
1991-05-01
Pages
3802-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51541
Subset
IM
Databases
GENBANK
M68844, M68845, M68846, M68847, M68848, M68849, M68850, M68851, M68852, X53027
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.88.9.3802 PMC Full Text View journal details
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