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PMID: 20525852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

The Plant cell ·Vol. 22 ·No. 6 ·2010-06-00 ·Pages 1936-46

Sugliani M, Brambilla V, Clerkx EJ, Koornneef M, Soppe WJ

Abstract

ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3-alpha and ABI3-beta, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-beta transcript accumulates at the end of seed maturation. The two ABI3 transcripts differ by the presence of a cryptic intron in ABI3-alpha, which is spliced out in ABI3-beta. The suppressor of abi3-5 (sua) mutant consistently restores wild-type seed features in the frameshift mutant abi3-5 but does not suppress other abi3 mutant alleles. SUA is a conserved splicing factor, homologous to the human protein RBM5, and reduces splicing of the cryptic ABI3 intron, leading to a decrease in ABI3-beta transcript. In the abi3-5 mutant, ABI3-beta codes for a functional ABI3 protein due to frameshift restoration.

MeSH Terms
Alternative Splicing Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Base Sequence Chromosome Mapping Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Introns Molecular Sequence Data Mutation Phylogeny RNA, Plant/genetics RNA-Binding Proteins/genetics,metabolism Transcription Factors
Chemicals
ABI3 protein, Arabidopsis Arabidopsis Proteins RNA, Plant RNA-Binding Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sugliani Matteo
Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany. sugliani@mpipz.mpg.de
Brambilla Vittoria
Clerkx Emile J M
Koornneef Maarten
Soppe Wim J J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.074674
Email
sugliani@mpipz.mpg.de
Published
2010-06-00
Epub
2010-00-04
Pages
1936-46
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2910958
Subset
IM
Databases
GENBANK
AAH43058, ADE44117, CBI33920, EEC72564, GU735482
RefSeq
NP_005769, NP_849543
External Links
PubMed source DOI: 10.1105/tpc.110.074674 PMC Full Text View journal details
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