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PMID: 10747033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A Sm-like protein complex that participates in mRNA degradation.

The EMBO journal ·Vol. 19 ·No. 7 ·2000-04-03 ·Pages 1661-71

Bouveret E, Rigaut G, Shevchenko A, Wilm M, Séraphin B

Abstract

In eukaryotes, seven Sm proteins bind to the U1, U2, U4 and U5 spliceosomal snRNAs while seven Smlike proteins (Lsm2p-Lsm8p) are associated with U6 snRNA. Another yeast Sm-like protein, Lsm1p, does not interact with U6 snRNA. Surprisingly, using the tandem affinity purification (TAP) method, we identified Lsm1p among the subunits associated with Lsm3p. Coprecipitation experiments demonstrated that Lsm1p, together with Lsm2p-Lsm7p, forms a new seven-subunit complex. We purified the two related Sm-like protein complexes and identified the proteins recovered in the purified preparations by mass spectrometry. This confirmed the association of the Lsm2p-Lsm8p complex with U6 snRNA. In contrast, the Lsm1p-Lsm7p complex is associated with Pat1p and Xrn1p exoribonuclease, suggesting a role in mRNA degradation. Deletions of LSM1, 6, 7 and PAT1 genes increased the half-life of reporter mRNAs. Interestingly, accumulating mRNAs were capped, suggesting a block in mRNA decay at the decapping step. These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming.

MeSH Terms
Codon, Nonsense/genetics Fungal Proteins/chemistry,genetics,metabolism Gene Deletion Genes, Fungal Genes, Reporter Macromolecular Substances RNA Caps/genetics,metabolism RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism Ribonucleoproteins, Small Nuclear/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Codon, Nonsense Fungal Proteins Macromolecular Substances RNA Caps RNA, Fungal RNA, Messenger Ribonucleoproteins, Small Nuclear
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bouveret E
EMBL, Meyerhofstrasse-1, D-69117 Heidelberg, Germany.
Rigaut G
Shevchenko A
Wilm M
Séraphin B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.7.1661
Published
2000-04-03
Pages
1661-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC310234
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.7.1661 PMC Full Text View journal details
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