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PMID: 20461056 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway.

The EMBO journal ·Vol. 29 ·No. 12 ·2010-06-16 ·Pages 1961-75

Khandelwal P, Ruiz WG, Apodaca G

Abstract

Compensatory endocytosis (CE) ensures recycling of membrane components and maintenance of plasma membrane size; however, the mechanisms, regulation, and physiological functions of clathrin-independent modes of CE are poorly understood. CE was studied in umbrella cells, which undergo regulated exocytosis of subapical discoidal/fusiform vesicles (DFV) during bladder filling, and may then replenish the pool of DFV by internalizing apical membrane during voiding. We found that voiding-stimulated CE, which depended on beta(1) integrin-associated signalling pathways, occurred by a dynamin-, actin-, and RhoA-regulated mechanism and was independent of caveolins, clathrin, and flotillin. Internalized apical membrane and fluid were initially found in ZO-1-positive vesicles, which were distinct from DFV, classical early endosomes, or the Golgi, and subsequently in lysosomes. We conclude that clathrin-independent CE in umbrella cells functions to recover membrane during voiding, is integrin regulated, occurs by a RhoA- and dynamin-dependent pathway, and terminates in degradation and not recapture of membrane in DFV.

MeSH Terms
Actins/metabolism Animals Caveolins/metabolism Clathrin/metabolism Dynamins/metabolism Endocytosis Integrin beta1/metabolism Membrane Proteins/metabolism Rabbits Transport Vesicles/physiology Urinary Bladder/physiology Urothelium/physiology rhoA GTP-Binding Protein/metabolism
Chemicals
Actins Caveolins Clathrin Integrin beta1 Membrane Proteins flotillins rhoA GTP-Binding Protein Dynamins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Khandelwal Puneet
Department of Medicine, Laboratory of Epithelial Cell Biology and Renal-Electrolyte Division, University of Pittsburgh, Pittsburgh, PA, USA.
Ruiz Wily G
Apodaca Gerard
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2010.91
Published
2010-06-16
Epub
2010-00-11
Pages
1961-75
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2892371
Subset
IM
Grants
NIDDK NIH HHS · R01-DK077777 · United States
NIDDK NIH HHS · P30DK079307 · United States
NIDDK NIH HHS · P30 DK079307 · United States
NIDDK NIH HHS · R37-DK54425 · United States
NIDDK NIH HHS · R37 DK054425 · United States
NIDDK NIH HHS · R01 DK077777 · United States
External Links
PubMed source DOI: 10.1038/emboj.2010.91 PMC Full Text View journal details
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