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PMID: 10684256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.

The Journal of cell biology ·Vol. 148 ·No. 4 ·2000-02-21 ·Pages 755-67

Smith RM, Baibakov B, Ikebuchi Y, White BH, Lambert NA, Kaczmarek LK, Vogel SS

Abstract

Proteins inserted into the cell surface by exocytosis are thought to be retrieved by compensatory endocytosis, suggesting that retrieval requires granule proteins. In sea urchin eggs, calcium influx through P-type calcium channels is required for retrieval, and the large size of sea urchin secretory granules permits the direct observation of retrieval. Here we demonstrate that retrieval is limited to sites of prior exocytosis. We tested whether channel distribution can account for the localization of retrieval at exocytotic sites. We find that P-channels reside on secretory granules before fertilization, and are translocated to the egg surface by exocytosis. Our study provides strong evidence that the transitory insertion of P-type calcium channels in the surface membrane plays an obligatory role in the mechanism coupling exocytosis and compensatory endocytosis.

MeSH Terms
Animals Binding Sites/drug effects Cadmium/pharmacology Calcimycin/pharmacology Calcium/metabolism,pharmacology Calcium Channel Blockers/metabolism,pharmacology Calcium Channels, P-Type/analysis,metabolism Cell Membrane/chemistry,drug effects,metabolism,ultrastructure Concanavalin A/metabolism Cytoplasmic Granules/chemistry,drug effects,metabolism,ultrastructure Endocytosis/drug effects Exocytosis/drug effects Fertilization/physiology Fluorescent Dyes/metabolism Membrane Potentials/drug effects,physiology Microscopy, Electron Ovum/cytology,drug effects,metabolism,ultrastructure Sea Urchins
Chemicals
Calcium Channel Blockers Calcium Channels, P-Type Fluorescent Dyes Cadmium Concanavalin A Calcimycin Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Smith R M
Medical College of Georgia, Augusta, Georgia 30912-2630, USA.
Baibakov B
Ikebuchi Y
White B H
Lambert N A
Kaczmarek L K
Vogel S S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.148.4.755
Published
2000-02-21
Pages
755-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2169375
Subset
IM
Grants
NINDS NIH HHS · NS36455 · United States
External Links
PubMed source DOI: 10.1083/jcb.148.4.755 PMC Full Text View journal details
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