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PMID: 10339572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 11 ·1999-05-25 ·Pages 6241-8

Cheong KH, Zacchetti D, Schneeberger EE, Simons K

Abstract

Apical proteins are sorted and delivered from the trans-Golgi network to the plasma membrane by a mechanism involving sphingolipid-cholesterol rafts. In this paper, we report the effects of changing the levels of VIP17/MAL, a tetraspan membrane protein localized to post-Golgi transport containers and the apical cell surface in MDCK cells. Overexpression of VIP17/MAL disturbed the morphology of the MDCK cell layers by increasing apical delivery and seemingly expanding the apical cell surface domains. On the other hand, expression of antisense RNA directed against VIP17/MAL caused accumulation in the Golgi and/or impaired apical transport of different apical protein markers, i.e., influenza virus hemagglutinin, the secretory protein clusterin (gp80), the transmembrane protein gp114, and a glycosylphosphatidylinositol-anchored protein. However, antisense RNA expression did not affect the distribution of E-cadherin to the basolateral surface. Because VIP17/MAL associates with sphingolipid-cholesterol rafts, these data provide functional evidence that this protein is involved in apical transport and might be a component of the machinery clustering lipid rafts with apical cargo to form apical transport carriers.

MeSH Terms
Animals Base Sequence Biological Transport Cadherins/genetics,metabolism Carrier Proteins/metabolism Cell Line Cell Membrane/metabolism Cricetinae Dogs Genes, Reporter Golgi Apparatus/metabolism Hemagglutinin Glycoproteins, Influenza Virus/genetics Humans Kidney Kinetics Membrane Proteins/genetics,metabolism Membrane Transport Proteins Molecular Sequence Data Myelin Proteins Myelin and Lymphocyte-Associated Proteolipid Proteins Oligodeoxyribonucleotides/chemistry Proteolipids/genetics,metabolism RNA, Antisense/genetics Transfection
Chemicals
Cadherins Carrier Proteins Hemagglutinin Glycoproteins, Influenza Virus MAL protein, human Membrane Proteins Membrane Transport Proteins Myelin Proteins Myelin and Lymphocyte-Associated Proteolipid Proteins Oligodeoxyribonucleotides Proteolipids RNA, Antisense
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cheong K H
European Molecular Biology Laboratory, Cell Biology and Biophysics Programme, D69012 Heidelberg and Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
Zacchetti D
Schneeberger E E
Simons K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.11.6241
Published
1999-05-25
Pages
6241-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26866
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.96.11.6241 PMC Full Text View journal details
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