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PMID: 7896870 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Annexin XIIIb: a novel epithelial specific annexin is implicated in vesicular traffic to the apical plasma membrane.

The Journal of cell biology ·Vol. 128 ·No. 6 ·1995-03-00 ·Pages 1043-53

Fiedler K, Lafont F, Parton RG, Simons K

Abstract

The sorting of apical and basolateral proteins into vesicular carriers takes place in the trans-Golgi network (TGN) in MDCK cells. We have previously analyzed the protein composition of immunoisolated apical and basolateral transport vesicles and have now identified a component that is highly enriched in apical vesicles. Isolation of the encoding cDNA revealed that this protein, annexin XIIIb, is a new isoform of the epithelial specific annexin XIII sub-family which includes the previously described intestine-specific annexin (annexin XIIIa; Wice, B. M., and J. I. Gordon. 1992. J. Cell Biol. 116:405-422). Annexin XIIIb differs from annexin XIIIa in that it contains a unique insert of 41 amino acids in the NH2 terminus and is exclusively expressed in dog intestine and kidney. Immunofluorescence microscopy demonstrated that annexin XIIIb was localized to the apical plasma membrane and underlying punctate structures. Since annexins have been suggested to play a role in membrane-membrane interactions in exocytosis and endocytosis, we investigated whether annexin XIIIb is involved in delivery to the apical cell surface. To this aim we used permeabilized MDCK cells and a cytosol-dependent in vitro transport assay. Antibodies specific for annexin XIIIb significantly inhibited the transport of influenza virus hemagglutinin from the TGN to the apical plasma membrane while the transport of vesicular stomatitis virus glycoprotein to the basolateral cell surface was unaffected. We propose that annexin XIIIb plays a role in vesicular transport to the apical plasma membrane in MDCK cells.

MeSH Terms
Amino Acid Sequence Animals Annexins/genetics,immunology,isolation & purification,metabolism Antibodies/pharmacology Base Sequence Biological Transport/drug effects Cell Membrane/metabolism Cells, Cultured Cloning, Molecular Cytoplasmic Granules/metabolism DNA, Complementary Dogs Epithelium/metabolism,ultrastructure Intestines Kidney Molecular Sequence Data Phylogeny Sequence Alignment
Chemicals
Annexins Antibodies DNA, Complementary
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fiedler K
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Lafont F
Parton R G
Simons K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.128.6.1043
Published
1995-03-00
Pages
1043-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120424
Subset
IM
Databases
GENBANK
X80208, X80209
External Links
PubMed source DOI: 10.1083/jcb.128.6.1043 PMC Full Text View journal details
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