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PMID: 20453848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells.

Nature cell biology ·Vol. 12 ·No. 6 ·2010-06-00 ·Pages 543-52

Mor A, Suliman S, Ben-Yishay R, Yunger S, Brody Y, Shav-Tal Y

Abstract

The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in comparison with that of protein transport. We have established a mammalian system that allowed the real-time visualization and quantification of large single mRNA-protein complexes (mRNPs) during export. The in vivo dynamics of bulk mRNP transport and export, from transcription to the nuclear pore complex (NPC), occurred within a 5-40 minute time frame, with no NPC pile-up. mRNP export was rapid (about 0.5 s) and kinetically faster than nucleoplasmic diffusion. Export inhibition demonstrated that mRNA-NPC interactions were independent of ongoing export. Nucleoplasmic transport dynamics of intron-containing and intronless mRNAs were similar, yet an intron did increase export efficiency. Here we provide visualization and analysis at the single mRNP level of the various steps in nuclear gene expression and the inter-chromatin tracks through which mRNPs diffuse, and demonstrate the kinetics of mRNP-NPC interactions and translocation.

MeSH Terms
Active Transport, Cell Nucleus/genetics Animals Biological Transport/genetics Cell Nucleus/genetics,metabolism Cells/metabolism Chromosomes/metabolism Introns Mammals/genetics,metabolism Nuclear Pore/genetics,metabolism Protein Transport/genetics RNA, Messenger/genetics,metabolism Ribonucleoproteins/metabolism
Chemicals
RNA, Messenger Ribonucleoproteins messenger ribonucleoprotein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mor Amir
The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan 52900, Israel.
Suliman Shimrit
Ben-Yishay Rakefet
Yunger Sharon
Brody Yehuda
Shav-Tal Yaron
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1476-4679
DOI
10.1038/ncb2056
Published
2010-06-00
Epub
2010-00-09
Pages
543-52
Language
English
Region
England
NLM ID
100890575
Subset
IM
Corrections
CommentIn
PMID: 20517300
CommentIn
PMID: 20627070
External Links
PubMed source DOI: 10.1038/ncb2056 View journal details
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