Abstract
Messenger ribonucleoprotein particles (mRNPs) move randomly within nucleoplasm before they exit from the nucleus. To further understand mRNP trafficking, we have studied the intranuclear movement of a specific mRNP, the BR2 mRNP, in salivary gland cells in Chironomus tentans. Their polytene nuclei harbor giant chromosomes separated by vast regions of nucleoplasm, which allows us to study mRNP mobility without interference of chromatin. The particles were fluorescently labeled with microinjected oligonucleotides (DNA or RNA) complementary to BR2 mRNA or with the RNA-binding protein hrp36, the C. tentans homologue of hnRNP A1. Using high-speed laser microscopy, we followed the intranuclear trajectories of single mRNPs and characterized their motion within the nucleoplasm. The Balbiani ring (BR) mRNPs moved randomly, but unexpectedly, in a discontinuous manner. When mobile, they diffused with a diffusion coefficient corresponding to their size. Between mobile phases, the mRNPs were slowed down 10- to 250-fold but were never completely immobile. Earlier electron microscopy work has indicated that BR particles can attach to thin nonchromatin fibers, which are sometimes connected to discrete fibrogranular clusters. We propose that the observed discontinuous movement reflects transient interactions between freely diffusing BR particles and these submicroscopic structures.
MeSH Terms
Animals
Biological Transport
Cell Nucleus/metabolism
Chironomidae
Chromatin
Diffusion
Insect Proteins/metabolism
Intracellular Fluid/metabolism
Ribonucleoproteins/metabolism
Salivary Glands/cytology
Chemicals
BR2.2 protein, Chironomus tentans
Chromatin
Insect Proteins
Ribonucleoproteins
messenger ribonucleoprotein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Siebrasse Jan Peter
Institute for Physical and Theoretical Chemistry, Wegelerstrasse 12, Rheinische Friedrich Wilhelms University Bonn, D-53115 Bonn, Germany.
Veith Roman
Dobay Akos
Leonhardt Heinrich
Daneholt Bertil
Kubitscheck Ulrich
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