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PMID: 20418883 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells.

Nature structural & molecular biology ·Vol. 17 ·No. 5 ·2010-05-00 ·Pages 635-40

Moqtaderi Z, Wang J, Raha D, White RJ, Snyder M, Weng Z, Struhl K

Abstract

Genome-wide occupancy profiles of five components of the RNA polymerase III (Pol III) machinery in human cells identified the expected tRNA and noncoding RNA targets and revealed many additional Pol III-associated loci, mostly near short interspersed elements (SINEs). Several genes are targets of an alternative transcription factor IIIB (TFIIIB) containing Brf2 instead of Brf1 and have extremely low levels of TFIIIC. Strikingly, expressed Pol III genes, unlike nonexpressed Pol III genes, are situated in regions with a pattern of histone modifications associated with functional Pol II promoters. TFIIIC alone associates with numerous ETC loci, via the B box or a novel motif. ETCs are often near CTCF binding sites, suggesting a potential role in chromosome organization. Our results suggest that human Pol III complexes associate preferentially with regions near functional Pol II promoters and that TFIIIC-mediated recruitment of TFIIIB is regulated in a locus-specific manner.

MeSH Terms
Base Sequence Cell Line DNA Polymerase II/genetics Genetic Loci Genome, Human Humans Promoter Regions, Genetic RNA Polymerase III/genetics,metabolism TATA-Binding Protein Associated Factors/genetics Transcription Factor TFIIIB/genetics Transcription Factors/genetics Transcription Factors, TFIII/genetics
Chemicals
BRF1 protein, human BRF2 protein, human TATA-Binding Protein Associated Factors Transcription Factor TFIIIB Transcription Factors Transcription Factors, TFIII transcription factor TFIIIC RNA Polymerase III DNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Moqtaderi Zarmik
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Wang Jie
Raha Debasish
White Robert J
Snyder Michael
Weng Zhiping
Struhl Kevin
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1794
Published
2010-05-00
Epub
2010-00-25
Pages
635-40
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC3350333
Subset
IM
Grants
NHGRI NIH HHS · HG 4695 · United States
NHGRI NIH HHS · R01 HG003110 · United States
NHGRI NIH HHS · HG 4558 · United States
NHGRI NIH HHS · U01 HG004695 · United States
NIGMS NIH HHS · GM 30186 · United States
NHGRI NIH HHS · U54 HG004558 · United States
NIGMS NIH HHS · R01 GM030186 · United States
NIGMS NIH HHS · R37 GM030186 · United States
External Links
PubMed source DOI: 10.1038/nsmb.1794 PMC Full Text View journal details
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