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PMID: 14592981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TFIIIB is phosphorylated, disrupted and selectively released from tRNA promoters during mitosis in vivo.

The EMBO journal ·Vol. 22 ·No. 21 ·2003-11-03 ·Pages 5841-50

Fairley JA, Scott PH, White RJ

Abstract

Mitosis involves a generalized repression of gene expression. In the case of RNA polymerase III transcription, this is due to phosphorylation-mediated inactivation of TFIIIB, an essential complex comprising the TATA-binding protein TBP and the TAF subunits Brf1 and Bdp1. In HeLa cells, this repression is mediated by a mitotic kinase other than cdc2-cyclin B and is antagonized by protein phosphatase 2A. Brf1 is hyperphosphorylated in metaphase-arrested cells, but remains associated with promoters in condensed chromosomes, along with TBP. In contrast, Bdp1 is selectively released. Repression can be reversed by raising the concentration of Brf1 or Bdp1. The data support a model in which hyperphosphorylation disrupts TFIIIB during mitosis, compromising its ability to support transcription.

MeSH Terms
CDC2 Protein Kinase/metabolism DNA Polymerase III/metabolism HeLa Cells Humans In Vitro Techniques Mitosis/genetics,physiology Models, Biological Phosphoprotein Phosphatases/metabolism Phosphorylation Promoter Regions, Genetic Protein Phosphatase 2 RNA, Transfer/genetics Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors/metabolism TATA-Box Binding Protein/metabolism Transcription Factor TFIIIB/metabolism
Chemicals
BRF1 protein, S cerevisiae BRF1 protein, human Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIIIB RNA, Transfer CDC2 Protein Kinase DNA Polymerase III Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fairley Jennifer A
Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK.
Scott Pamela H
White Robert J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg544
Published
2003-11-03
Pages
5841-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC275401
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg544 PMC Full Text View journal details
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