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PMID: 20403812 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence-specific recognition of methylated DNA by human zinc-finger proteins.

Nucleic acids research ·Vol. 38 ·No. 15 ·2010-08-00 ·Pages 5015-22

Sasai N, Nakao M, Defossez PA

Abstract

DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.

MeSH Terms
Base Sequence Binding Sites Consensus Sequence DNA Methylation DNA-Binding Proteins/chemistry,metabolism Humans Protein Binding Repressor Proteins/chemistry,metabolism Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Repressor Proteins Transcription Factors ZBTB33 protein, human ZBTB38 protein, human ZBTB4 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sasai Nobuhiro
CNRS UMR7216, Université Paris-Diderot, Paris, France.
Nakao Mitsuyoshi
Defossez Pierre-Antoine
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkq280
Published
2010-08-00
Epub
2010-00-19
Pages
5015-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2926618
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkq280 PMC Full Text View journal details
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