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PMID: 20305652 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Direct determination of molecular haplotypes by chromosome microdissection.

Nature methods ·Vol. 7 ·No. 4 ·2010-04-00 ·Pages 299-301

Ma L, Xiao Y, Huang H, Wang Q, Rao W, Feng Y, Zhang K, Song Q

Abstract

Direct observation of haplotypes is still technically challenging. Here we report a method for the determination of haplotypes through chromosome microdissection. We determined human long-range chromosomal haplotypes with more than 98.85% accuracy at 24,245 genome-wide heterozygous single-nucleotide polymorphism (SNP) loci.

MeSH Terms
Chromosome Mapping/methods Female Genome, Human Haplotypes Humans Male Microdissection/methods Polymorphism, Single Nucleotide
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ma Li
Cardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA.
Xiao Yan
Huang Hui
Wang Qingwei
Rao Weinian
Feng Yue
Zhang Kui
Song Qing
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14 references, click to expand
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.1443
Published
2010-04-00
Epub
2010-00-21
Pages
299-301
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2871314
Subset
IM
Grants
NHLBI NIH HHS · UH1 HL003676-07 · United States
NCRR NIH HHS · G12 RR003034-235447 · United States
NHLBI NIH HHS · R25 HL003676 · United States
NHGRI NIH HHS · R21 HG006173 · United States
NIDDK NIH HHS · P30 DK072488 · United States
NHLBI NIH HHS · UH1 HL003676 · United States
NHGRI NIH HHS · U01 HG004436 · United States
NINDS NIH HHS · R01 NS045012-02 · United States
NHGRI NIH HHS · HG004436 · United States
NCRR NIH HHS · U54 RR022814-019005 · United States
NIGMS NIH HHS · S06 GM008248-160056 · United States
NIGMS NIH HHS · R01 GM074913-04 · United States
NCRR NIH HHS · U54 RR014758-05S10003 · United States
NHLBI NIH HHS · SC2 HL095098-01 · United States
NINDS NIH HHS · R01 NS045012 · United States
NHLBI NIH HHS · SC2 HL095098 · United States
NCRR NIH HHS · RR014758 · United States
NHGRI NIH HHS · U01 HG004436-02 · United States
NIGMS NIH HHS · GM074913 · United States
NCRR NIH HHS · U54 RR022814-04S17179 · United States
NCRR NIH HHS · G12 RR003034 · United States
NCRR NIH HHS · U54 RR014758 · United States
NHLBI NIH HHS · T32 HL067702-09 · United States
NCRR NIH HHS · U54 RR022814-046880 · United States
NCRR NIH HHS · U54 RR022814 · United States
NIGMS NIH HHS · R01 GM074913 · United States
NCRR NIH HHS · G12 RR003032-130001 · United States
NCRR NIH HHS · U54 RR014758-01 · United States
NCRR NIH HHS · RR003034 · United States
NIGMS NIH HHS · S06 GM008248 · United States
NHLBI NIH HHS · R25 HL003676-12 · United States
NCRR NIH HHS · U54 RR022814-035966 · United States
NHLBI NIH HHS · HL003676 · United States
NHLBI NIH HHS · T32 HL067702 · United States
NCRR NIH HHS · U54 RR022814-026316 · United States
NINDS NIH HHS · NS45012 · United States
External Links
PubMed source DOI: 10.1038/nmeth.1443 PMC Full Text View journal details
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