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PMID: 2022181 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Constitutive expression of nitrate reductase allows normal growth and development of Nicotiana plumbaginifolia plants.

The EMBO journal ·Vol. 10 ·No. 5 ·1991-05-00 ·Pages 1027-35

Vincentz M, Caboche M

Abstract

A nitrate reductase (NR) deficient mutant of Nicotiana plumbaginifolia totally impaired in the production of NR transcript and protein was restored for NR activity by transformation with a chimaeric NR gene. This gene was composed of a full-length tobacco NR cDNA fused to the CaMV 35S promoter and to termination signals from the tobacco NR gene. The transgenic plants we obtained were viable and fertile and expressed from one-fifth to three times the wild-type NR activity in their leaves. The analysis of chimeric NR gene expression in these plants showed, by comparison with wild-type plants, that the regulation of NR gene expression by light, nitrate and circadian rhythm takes place at the transcriptional level. However, unlike nitrate, light was required for the accumulation of NR protein in transgenic plants, suggesting that NR expression is also controlled at the translational and/or post-translational level.

Related Genes
NR
MeSH Terms
Chimera Circadian Rhythm Darkness Genetic Complementation Test Lighting Mutation Nitrate Reductase Nitrate Reductases/biosynthesis,genetics Nucleotide Mapping Plants, Toxic Promoter Regions, Genetic Protein Processing, Post-Translational RNA, Messenger/metabolism Terminator Regions, Genetic Tobacco/enzymology,growth & development Transformation, Genetic
Chemicals
RNA, Messenger Nitrate Reductases Nitrate Reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vincentz M
Laboratoire de Biologie cellulaire, INRA, Centre de Versailles, France.
Caboche M
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-05-00
Pages
1027-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452753
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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