Home LiteratureArticle Details
PMID: 2020545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and sequence determination of the host factor gene for bacteriophage Q beta.

Nucleic acids research ·Vol. 19 ·No. 5 ·1991-03-11 ·Pages 1063-6

Kajitani M, Ishihama A

Abstract

The host factor (HF-I) required for phage Q beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues.

Related Genes
hfq
MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/genetics,isolation & purification Cloning, Molecular Coliphages/genetics DNA, Bacterial DNA-Binding Proteins/genetics,isolation & purification Escherichia coli/genetics Escherichia coli Proteins Genes, Bacterial Host Factor 1 Protein Integration Host Factors Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Carrier Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Host Factor 1 Protein Integration Host Factors integration host factor, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kajitani M
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Ishihama A
References (19)
19 references, click to expand
  1. Resolution of two factors required in the Q-beta-RNA polymerase reaction.
    Nature. 1968 Nov 2;220(5166):478-80 PMID: 4879600
  2. Three cistrons in bacteriophage Q beta.
    Virology. 1970 Sep;42(1):49-60 PMID: 4918276
  3. Subunit structure of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):525-7 PMID: 5472467
  4. Characterization of the subunits of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):527-33 PMID: 5472468
  5. Grouping of RNA phages based on the template specificity of their RNA replicases.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2022-4 PMID: 5289361
  6. In vitro replication of bacteriophage GA RNA. Involvement of host factor(s) in GA RNA replication.
    J Biochem. 1981 Mar;89(3):751-7 PMID: 6169703
  7. DNA sequence and transcript mapping of MOD5: features of the 5' region which suggest two translational starts.
    Mol Cell Biol. 1987 Jan;7(1):185-91 PMID: 3031457
  8. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  9. Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.
    J Bacteriol. 1987 Sep;169(9):4076-85 PMID: 3040675
  10. Proteins from the prokaryotic nucleoid: primary and quaternary structure of the 15-kD Escherichia coli DNA binding protein H-NS.
    Mol Microbiol. 1988 May;2(3):323-9 PMID: 3135462
  11. Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS.
    Mol Gen Genet. 1988 May;212(2):199-202 PMID: 2841565
  12. Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12.
    J Bacteriol. 1989 Jun;171(6):3233-46 PMID: 2656644
  13. Factor fraction required for the synthesis of bacteriophage Qbeta-RNA.
    Nature. 1968 Aug 10;219(5154):588-90 PMID: 4874917
  14. Bacterial proteins required for replication of phage Q ribonucleic acid. Pruification and properties of host factor I, a ribonucleic acid-binding protein.
    J Biol Chem. 1972 Feb 10;247(3):824-31 PMID: 4550762
  15. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7 PMID: 4624757
  16. Cistron specific translation control protein in Escherichia coli.
    Nat New Biol. 1972 Sep 6;239(88):16-9 PMID: 4562228
  17. Host subunit of Q replicase is translation control factor i.
    Nat New Biol. 1972 Sep 6;239(88):19-20 PMID: 4562229
  18. The host factor required for RNA phage Qbeta RNA replication in vitro. Intracellular location, quantitation, and purification by polyadenylate-cellulose chromatography.
    J Biol Chem. 1975 May 25;250(10):3607-612 PMID: 805130
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.5.1063
Published
1991-03-11
Pages
1063-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333781
Subset
IM
Databases
GENBANK
D00743, S62405, S62419, S62424, X56310, X56998, X56999, X57453, X57454, X57455
External Links
PubMed source DOI: 10.1093/nar/19.5.1063 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2