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PMID: 20190228 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Redundant control of adipogenesis by histone deacetylases 1 and 2.

The Journal of biological chemistry ·Vol. 285 ·No. 19 ·2010-05-07 ·Pages 14663-70

Haberland M, Carrer M, Mokalled MH, Montgomery RL, Olson EN

Abstract

Adipocyte differentiation is a well defined process that is under the control of transcriptional activators and repressors. We show that histone deacetylase (HDAC) inhibitors efficiently block adipocyte differentiation in vitro. This effect is specific to adipogenesis, as another mesenchymal differentiation process, osteoblastogenesis, is enhanced upon HDAC inhibition. Through the systematic genetic deletion of HDAC genes in cultured mesenchymal precursor cells, we show that deletion of HDAC1 and HDAC2 leads to reduced lipid accumulation, revealing redundant and requisite roles of these class I HDACs in adipogenesis. These findings unveil a previously unrecognized role for HDACs in the control of adipogenesis.

MeSH Terms
3T3-L1 Cells Adipogenesis/drug effects,physiology Animals Biomarkers/metabolism Blotting, Western Butyrates/pharmacology Cell Differentiation Cells, Cultured Embryo, Mammalian/cytology,drug effects,metabolism Fibroblasts/cytology,drug effects,metabolism Fluorescent Antibody Technique, Indirect Gene Expression Profiling Histone Deacetylase 1/antagonists & inhibitors,genetics,metabolism Histone Deacetylase 2/antagonists & inhibitors,genetics,metabolism Histone Deacetylase Inhibitors/pharmacology Mesenchymal Stem Cells/cytology,drug effects,metabolism Mice Oligonucleotide Array Sequence Analysis Osteogenesis PPAR gamma/metabolism RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Biomarkers Butyrates Histone Deacetylase Inhibitors PPAR gamma RNA, Messenger Hdac1 protein, mouse Hdac2 protein, mouse Histone Deacetylase 1 Histone Deacetylase 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haberland Michael
Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9148, USA.
Carrer Michele
Mokalled Mayssa H
Montgomery Rusty L
Olson Eric N
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M109.081679
Published
2010-05-07
Epub
2010-00-26
Pages
14663-70
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2863240
Subset
IM
Grants
NHLBI NIH HHS · R01 HL083371 · United States
NHLBI NIH HHS · R01 HL093039 · United States
NHLBI NIH HHS · 5-R01-HL083371 · United States
External Links
PubMed source DOI: 10.1074/jbc.M109.081679 PMC Full Text View journal details
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