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PMID: 20156967 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Unconventional secretion of Acb1 is mediated by autophagosomes.

The Journal of cell biology ·Vol. 188 ·No. 4 ·2010-02-22 ·Pages 527-36

Duran JM, Anjard C, Stefan C, Loomis WF, Malhotra V

Abstract

Starving Dictyostelium discoideum cells secrete AcbA, an acyl coenzyme A-binding protein (ACBP) that lacks a conventional signal sequence for entering the endoplasmic reticulum (ER). Secretion of AcbA in D. discoideum requires the Golgi-associated protein GRASP. In this study, we report that starvation-induced secretion of Acb1, the Saccharomyces cerevisiae ACBP orthologue, also requires GRASP (Grh1). This highlights the conserved function of GRASP in unconventional secretion. Although genes required for ER to Golgi or Golgi to cell surface transport are not required for Acb1 secretion in yeast, this process involves autophagy genes and the plasma membrane t-SNARE, Sso1. Inhibiting transport to vacuoles does not affect Acb1 secretion. In sum, our experiments reveal a unique secretory pathway where autophagosomes containing Acb1 evade fusion with the vacuole to prevent cargo degradation. We propose that these autophagosome intermediates fuse with recycling endosomes instead to form multivesicular body carriers that then fuse with the plasma membrane to release cargo.

MeSH Terms
Amino Acid Sequence Autophagy/genetics Carrier Proteins/chemistry,metabolism Cell Membrane/metabolism Genes, Fungal/genetics Golgi Apparatus/metabolism Intercellular Signaling Peptides and Proteins Membrane Fusion Models, Biological Molecular Sequence Data Multivesicular Bodies/metabolism Peptides/metabolism Phagosomes/metabolism Qa-SNARE Proteins/metabolism Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism Secretory Pathway Vacuoles/metabolism
Chemicals
ACB1 protein, S cerevisiae Carrier Proteins Intercellular Signaling Peptides and Proteins Peptides Qa-SNARE Proteins SDF-2 protein, Dictyostelium discoideum SSO1 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Duran Juan M
Center for Genomic Regulation, Barcelona 08003, Spain.
Anjard Christophe
Stefan Chris
Loomis William F
Malhotra Vivek
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200911154
Published
2010-02-22
Epub
2010-00-15
Pages
527-36
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2828925
Subset
IM
Grants
NIGMS NIH HHS · R01 GM078175 · United States
NIGMS NIH HHS · GM78175 · United States
Corrections
CommentIn
PMID: 20156968
External Links
PubMed source DOI: 10.1083/jcb.200911154 PMC Full Text View journal details
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