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PMID: 2967495 Published · ppublish English Comparative Study Journal Article

Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 11 ·1988-06-00 ·Pages 3708-12

Grundmann U, Abel KJ, Bohn H, Löbermann H, Lottspeich F, Küpper H

Abstract

A cDNA library prepared from human placenta was screened for sequences encoding the placental protein 4 (PP4). PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Partial amino acid sequence information from PP4-derived cyanogen bromide fragments was used to design three oligonucleotide probes for screening the library. From 10(6)independent recombinants, 18 clones were identified that hybridized to all three probes. These 18 recombinants contained cDNA inserts encoding a protein of 320 amino acid residues. In addition to the PP4 cDNA we identified 9 other recombinants encoding a protein with considerable similarity (74%) TO PP4, which was termed PP4-X. PP4 and PP4-X belong to the lipocortin family, as judged by their homology to lipocortin I and calpactin I.

MeSH Terms
Amino Acid Sequence Annexin A5 Annexins Base Sequence Calcium-Binding Proteins Cloning, Molecular DNA/genetics Glycoproteins Humans Pregnancy Proteins/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid Thromboplastin/antagonists & inhibitors
Chemicals
Annexin A5 Annexins Calcium-Binding Proteins Glycoproteins Pregnancy Proteins RNA, Messenger DNA Thromboplastin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Grundmann U
Department of Molecular Biology, Research Institutes, Marburg, Federal Republic of Germany.
Abel K J
Bohn H
Löbermann H
Lottspeich F
Küpper H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.11.3708
Published
1988-06-00
Pages
3708-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280287
Subset
IM
Databases
GENBANK
J03264, M19384
External Links
PubMed source DOI: 10.1073/pnas.85.11.3708 PMC Full Text View journal details
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