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PMID: 20118937 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae.

Nature structural & molecular biology ·Vol. 17 ·No. 2 ·2010-02-00 ·Pages 244-7

Hu W, Petzold C, Coller J, Baker KE

Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5' end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

MeSH Terms
Codon, Nonsense Endoribonucleases/metabolism Polyribosomes/metabolism RNA Stability RNA, Messenger/metabolism Saccharomyces cerevisiae/metabolism
Chemicals
Codon, Nonsense RNA, Messenger mRNA decapping enzymes Endoribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hu Wenqian
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA.
Petzold Christine
Coller Jeff
Baker Kristian E
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1734
Published
2010-02-00
Epub
2010-00-31
Pages
244-7
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2840703
Subset
IM
Grants
NIGMS NIH HHS · R01 GM080465 · United States
NIGMS NIH HHS · R01 GM080465-02 · United States
NIGMS NIH HHS · GM080465 · United States
NIGMS NIH HHS · R01 GM080465-01 · United States
NIGMS NIH HHS · R01 GM080465-04 · United States
NIGMS NIH HHS · R01 GM080465-03 · United States
NIGMS NIH HHS · R01 GM080465-03S1 · United States
NIGMS NIH HHS · R01 GM080465-05 · United States
External Links
PubMed source DOI: 10.1038/nsmb.1734 PMC Full Text View journal details
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