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PMID: 2011520 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and sequence determination of the Schizosaccharomyces pombe rpb1 gene encoding the largest subunit of RNA polymerase II.

Nucleic acids research ·Vol. 19 ·No. 3 ·1991-02-11 ·Pages 461-8

Azuma Y, Yamagishi M, Ueshima R, Ishihama A

Abstract

The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.

Related Genes
rpb1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular DNA, Fungal/genetics Genes, Fungal Molecular Sequence Data RNA Polymerase II/genetics RNA, Fungal/genetics Restriction Mapping Schizosaccharomyces/enzymology,genetics Schizosaccharomyces pombe Proteins/genetics
Chemicals
DNA, Fungal RNA, Fungal Schizosaccharomyces pombe Proteins RNA Polymerase II Rpb1 protein, S pombe
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Azuma Y
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Yamagishi M
Ueshima R
Ishihama A
References (50)
50 references, click to expand
  1. Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III.
    Nucleic Acids Res. 1988 Sep 26;16(18):8753-72 PMID: 3174432
  2. Structure and function of bacterial sigma factors.
    Annu Rev Biochem. 1988;57:839-72 PMID: 3052291
  3. Transcription by RNA polymerase III.
    Annu Rev Biochem. 1988;57:873-914 PMID: 3052292
  4. Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Oct;8(10):3997-4008 PMID: 3054507
  5. Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain.
    Cell. 1989 Feb 24;56(4):585-97 PMID: 2917367
  6. The scanning model for translation: an update.
    J Cell Biol. 1989 Feb;108(2):229-41 PMID: 2645293
  7. Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus.
    J Cell Biol. 1989 Feb;108(2):243-53 PMID: 2537310
  8. In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure.
    Cell. 1989 Mar 10;56(5):815-27 PMID: 2924350
  9. Mutations in RNA polymerase II enhance or suppress mutations in GAL4.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2794-8 PMID: 2495535
  10. Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila.
    Mol Gen Genet. 1989 Jan;215(2):266-75 PMID: 2496296
  11. Cloning and sequence determination of the gene encoding the largest subunit of the fission yeast Schizosaccharomyces pombe RNA polymerase I.
    Gene. 1988 Dec 30;74(2):503-15 PMID: 2854522
  12. Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei.
    FEBS Lett. 1989 May 22;249(1):123-8 PMID: 2542092
  13. Promoter selectivity of prokaryotic RNA polymerases.
    Trends Genet. 1988 Oct;4(10):282-6 PMID: 3076288
  14. RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth.
    Mol Cell Biol. 1989 Jul;9(7):2854-9 PMID: 2674672
  15. Molecular characterization of the Trypanosoma brucei RNA polymerase I and III largest subunit genes.
    J Biol Chem. 1989 Oct 25;264(30):18091-9 PMID: 2808366
  16. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II.
    Mol Cell Biol. 1989 Oct;9(10):4119-30 PMID: 2586513
  17. RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus.
    Mol Cell Biol. 1989 Dec;9(12):5387-94 PMID: 2685562
  18. An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains.
    Nucleic Acids Res. 1989 Dec 11;17(23):9621-36 PMID: 2690004
  19. Unified approach to alignment and phylogenies.
    Methods Enzymol. 1990;183:626-45 PMID: 2314296
  20. Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method.
    Biotechniques. 1989 May;7(5):494-6, 498-9 PMID: 2483818
  21. Subunits shared by eukaryotic nuclear RNA polymerases.
    Genes Dev. 1990 Mar;4(3):313-23 PMID: 2186966
  22. RNA polymerase B (II) and general transcription factors.
    Annu Rev Biochem. 1990;59:711-54 PMID: 2197989
  23. RNA polymerase II subunit RPB10 is essential for yeast cell viability.
    J Biol Chem. 1990 Oct 15;265(29):17816-9 PMID: 2211663
  24. RNA polymerase II C-terminal repeat influences response to transcriptional enhancer signals.
    Nature. 1990 Oct 4;347(6292):491-4 PMID: 2215664
  25. RNA polymerase II: subunit structure and function.
    Trends Biochem Sci. 1990 Sep;15(9):347-51 PMID: 1700503
  26. Tails of RNA polymerase II.
    Trends Biochem Sci. 1990 Oct;15(10):383-7 PMID: 2251729
  27. Homologous domains of the largest subunit of eucaryotic RNA polymerase II are conserved in plants.
    Mol Gen Genet. 1990 Aug;223(1):65-75 PMID: 2259344
  28. Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1029-33 PMID: 772676
  29. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  30. Genetics of bacterial RNA polymerases.
    Annu Rev Genet. 1979;13:59-97 PMID: 94253
  31. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.
    Cell. 1984 Dec;39(3 Pt 2):611-21 PMID: 6096014
  32. Eukaryotic RNA polymerases.
    CRC Crit Rev Biochem. 1985;18(1):31-90 PMID: 3893883
  33. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.
    Cell. 1985 Sep;42(2):599-610 PMID: 3896517
  34. Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.
    Cell. 1985 Sep;42(2):611-21 PMID: 2992806
  35. Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence.
    Nature. 1985 Nov 7-13;318(6041):78-80 PMID: 2997624
  36. The regulation of transcription initiation in bacteria.
    Annu Rev Genet. 1985;19:355-87 PMID: 3936407
  37. Transcription of the triose-phosphate-isomerase gene of Schizosaccharomyces pombe initiates from a start point different from that in Saccharomyces cerevisiae.
    Gene. 1985;40(1):125-30 PMID: 3912263
  38. Splicing of messenger RNA precursors.
    Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
  39. Transcription signals and factors in Escherichia coli.
    Adv Biophys. 1986;21:163-73 PMID: 3529848
  40. Localization of an alpha-amanitin resistance mutation in the gene encoding the largest subunit of mouse RNA polymerase II.
    Mol Cell Biol. 1987 Feb;7(2):586-94 PMID: 3821724
  41. Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1192-6 PMID: 3547406
  42. Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.
    Mol Cell Biol. 1987 May;7(5):1602-11 PMID: 3299050
  43. Isolation and characterization of temperature-sensitive RNA polymerase II mutants of Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Jun;7(6):2155-64 PMID: 3299061
  44. Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element.
    EMBO J. 1987 Jun;6(6):1757-63 PMID: 3649292
  45. Cloning and sequence analysis of the mouse genomic locus encoding the largest subunit of RNA polymerase II.
    J Biol Chem. 1987 Aug 5;262(22):10695-705 PMID: 3038894
  46. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II.
    Cell. 1987 Sep 11;50(6):909-15 PMID: 3304659
  47. RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase.
    J Mol Biol. 1987 Jun 20;195(4):929-37 PMID: 3116266
  48. The C-terminal domain of the largest subunit of RNA polymerase II of Saccharomyces cerevisiae, Drosophila melanogaster, and mammals: a conserved structure with an essential function.
    Mol Cell Biol. 1988 Jan;8(1):321-9 PMID: 3122024
  49. Genetic analysis of the repetitive carboxyl-terminal domain of the largest subunit of mouse RNA polymerase II.
    Mol Cell Biol. 1988 Jan;8(1):330-9 PMID: 3275873
  50. RPA190, the gene coding for the largest subunit of yeast RNA polymerase A.
    J Biol Chem. 1988 Feb 25;263(6):2830-9 PMID: 2830265
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.3.461
Published
1991-02-11
Pages
461-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333634
Subset
IM
Databases
GENBANK
X56564, X60807, X60808, X60809, X60810, X60811, X60812, X60813, X60814, X60815
External Links
PubMed source DOI: 10.1093/nar/19.3.461 PMC Full Text View journal details
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