Home LiteratureArticle Details
PMID: 2009864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Double-strand-break repair and recombination catalyzed by a nuclear extract of Saccharomyces cerevisiae.

The EMBO journal ·Vol. 10 ·No. 4 ·1991-04-00 ·Pages 987-96

Symington LS

Abstract

An in vitro system for double-strand-break repair and recombination of plasmid substrates catalyzed by extracts prepared from yeast nuclei has been developed. Recombination events that generate crossover products were detected amongst reaction products by Southern blot hybridization, or by the polymerase chain reaction (PCR). The recombination reaction was found to be stimulated by a double-strand break within homologous sequences and proceeded by a mechanism that involved branched DNA intermediates. In addition to pairing events that generate crossovers, the formation of inverted repeats (head-to-head and tail-to-tail joined products) was also detected. Two models are presented which propose that the formation of crossover products and inverted repeats occur by similar mechanisms.

MeSH Terms
Base Sequence Blotting, Southern Cell Nucleus/metabolism Cloning, Molecular Crossing Over, Genetic DNA Damage DNA Repair Escherichia coli/genetics Models, Genetic Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plasmids Polymerase Chain Reaction Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Oligonucleotide Probes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Symington L S
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, NY 10032.
References (39)
39 references, click to expand
  1. Reversal of bacteriophage T4 induced polynucleotide kinase action.
    Biochemistry. 1973 Dec 4;12(25):5050-5 PMID: 4366306
  2. A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae.
    Cell. 1990 Jun 15;61(6):1089-101 PMID: 2190690
  3. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  5. Nucleosome organization of the yeast 2-micrometer DNA plasmid: a eukaryotic minichromosome.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6515-9 PMID: 392520
  6. Yeast transformation: a model system for the study of recombination.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
  7. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  8. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus.
    Cell. 1982 Nov;31(1):183-92 PMID: 6297747
  9. Separation of branched from linear DNA by two-dimensional gel electrophoresis.
    Anal Biochem. 1983 Apr 15;130(2):527-35 PMID: 6869840
  10. Yeast recombination: the association between double-strand gap repair and crossing-over.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4417-21 PMID: 6308623
  11. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  12. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  13. A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae.
    Cell. 1983 Nov;35(1):167-74 PMID: 6313222
  14. Genetic recombination of homologous plasmids catalyzed by cell-free extracts of Saccharomyces cerevisiae.
    Cell. 1983 Dec;35(3 Pt 2):805-13 PMID: 6360380
  15. Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
    Mol Cell Biol. 1984 Jun;4(6):1020-34 PMID: 6330525
  16. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  17. Transformation of yeast with linearized plasmid DNA. Formation of inverted dimers and recombinant plasmid products.
    J Mol Biol. 1985 Aug 5;184(3):375-87 PMID: 3900413
  18. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
    Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
  19. Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3248-52 PMID: 3517855
  20. Plasmid recombination intermediates generated in a Saccharomyces cerevisiae cell-free recombination system.
    Mol Cell Biol. 1985 Sep;5(9):2361-8 PMID: 3915541
  21. Double-strand breaks can initiate meiotic recombination in S. cerevisiae.
    Cell. 1986 Aug 29;46(5):733-40 PMID: 3527443
  22. Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.
    Mol Cell Biol. 1985 Aug;5(8):2080-9 PMID: 3018550
  23. A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7831-5 PMID: 3020559
  24. Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
    Mol Cell Biol. 1986 Jun;6(6):2053-61 PMID: 2946937
  25. Intramolecular recombination between transfected repeated sequences in mammalian cells is nonconservative.
    Mol Cell Biol. 1986 Jul;6(7):2520-6 PMID: 3023937
  26. Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5560-4 PMID: 3303029
  27. The localization of replication origins on ARS plasmids in S. cerevisiae.
    Cell. 1987 Nov 6;51(3):463-71 PMID: 2822257
  28. Identification of homologous pairing and strand-exchange activity from a human tumor cell line based on Z-DNA affinity chromatography.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):36-40 PMID: 2963327
  29. Intra-chromosomal gene conversion induced by a DNA double-strand break in Saccharomyces cerevisiae.
    J Mol Biol. 1988 May 20;201(2):247-60 PMID: 3047399
  30. The structure of the Holliday junction, and its resolution.
    Cell. 1988 Oct 7;55(1):79-89 PMID: 3167979
  31. Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences.
    Mol Cell Biol. 1988 Sep;8(9):3918-28 PMID: 3065627
  32. An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.
    Nature. 1989 Mar 2;338(6210):35-9 PMID: 2537472
  33. Double-strand breaks at an initiation site for meiotic gene conversion.
    Nature. 1989 Mar 2;338(6210):87-90 PMID: 2645528
  34. Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
    Genetics. 1989 Jul;122(3):519-34 PMID: 2668114
  35. Double-strand breaks stimulate alternative mechanisms of recombination repair.
    J Mol Biol. 1989 Jun 5;207(3):527-41 PMID: 2668534
  36. Intermolecular recombination between DNAs introduced into mouse L cells is mediated by a nonconservative pathway that leads to crossover products.
    Mol Cell Biol. 1990 Jan;10(1):103-12 PMID: 2294396
  37. Synapsis-mediated fusion of free DNA ends forms inverted dimer plasmids in yeast.
    Genetics. 1990 Jan;124(1):67-80 PMID: 2407606
  38. Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.
    EMBO J. 1990 Mar;9(3):663-73 PMID: 2178924
  39. Kinetics of branch migration in double-stranded DNA.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2299-303 PMID: 1065879
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-04-00
Pages
987-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452743
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041784 · United States
NIGMS NIH HHS · GM41784 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2