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PMID: 2009861 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Retinoblastoma cancer suppressor gene product is a substrate of the cell cycle regulator cdc2 kinase.

The EMBO journal ·Vol. 10 ·No. 4 ·1991-04-00 ·Pages 857-64

Lin BT, Gruenwald S, Morla AO, Lee WH, Wang JY

Abstract

The retinoblastoma gene product (RB) is a nuclear protein which has been shown to function as a tumor suppressor. It is phosphorylated from S to M phase of the cell cycle and dephosphorylated in G1. This suggests that the function of RB is regulated by its phosphorylation in the cell cycle. Ten phosphotryptic peptides are found in human RB proteins. The pattern of RB phosphorylation does not change from S to M phases of the cell cycle. Hypophosphorylated RB prepared from insect cells infected with an RB-recombinant baculovirus is used as a substrate for in vitro phosphorylation reactions. Of several protein kinases tested, only cdc2 kinase phosphorylates RB efficiently and all 10 peptides can be phosphorylated by cdc2 in vitro. Removal of cdc2 from mitotic cell extracts by immunoprecipitation causes a concomitant depletion of RB kinase activity. These results indicate that cdc2 or a kinase with similar substrate specificity is involved in the cell cycle-dependent phosphorylation of the RB protein.

MeSH Terms
CDC2 Protein Kinase/metabolism Cell Cycle Cell Line Genes, Retinoblastoma HeLa Cells/cytology,metabolism Humans Mitosis Nuclear Proteins/genetics,metabolism Peptide Mapping Phosphopeptides/isolation & purification Phosphorylation Retinoblastoma Protein/genetics,metabolism
Chemicals
Nuclear Proteins Phosphopeptides Retinoblastoma Protein CDC2 Protein Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lin B T
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Gruenwald S
Morla A O
Lee W H
Wang J Y
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-04-00
Pages
857-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452726
Subset
IM
Grants
NCI NIH HHS · R01 CA058320 · United States
NCI NIH HHS · CA 43045 · United States
NCI NIH HHS · CA 51495 · United States
External Links
PubMed source PMC Full Text View journal details
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