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PMID: 20097869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis.

The Plant cell ·Vol. 22 ·No. 1 ·2010-01-00 ·Pages 34-47

Baubec T, Dinh HQ, Pecinka A, Rakic B, Rozhon W, Wohlrab B, von Haeseler A, Mittelsten Scheid O

Abstract

Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case of transcriptional gene silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations or inhibitor treatments that activate many other suppressed genes. This raised the question about the molecular basis of this extreme stability. A combination of forward and reverse genetics and drug application provides evidence for an epigenetic double lock that is only alleviated upon the simultaneous removal of both DNA methylation and histone methylation. Therefore, the cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states and contributes to heritable diversity of gene expression patterns.

MeSH Terms
Adenosylhomocysteinase/genetics Alleles Arabidopsis/genetics Arabidopsis Proteins/genetics Chromatin/metabolism DNA Methylation DNA, Bacterial/genetics DNA, Plant/metabolism DNA-Binding Proteins/genetics Epigenesis, Genetic Gene Expression Regulation, Plant Histone Deacetylase Inhibitors/metabolism Histones/metabolism Mutagenesis, Insertional Mutation Polyploidy Sequence Analysis, DNA Transcription Factors/genetics Transcription, Genetic
Chemicals
Arabidopsis Proteins Chromatin DDM1 protein, Arabidopsis DNA, Bacterial DNA, Plant DNA-Binding Proteins Histone Deacetylase Inhibitors Histones T-DNA Transcription Factors Adenosylhomocysteinase HOG1 protein, Arabidopsis
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Baubec Tuncay
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, 1030 Viena, Austria.
Dinh Huy Q
Pecinka Ales
Rakic Branislava
Rozhon Wilfried
Wohlrab Bonnie
von Haeseler Arndt
Mittelsten Scheid Ortrun
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.109.072819
Published
2010-01-00
Epub
2010-00-22
Pages
34-47
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2828703
Subset
IM
Grants
Austrian Science Fund FWF · P 18986 · Austria
External Links
PubMed source DOI: 10.1105/tpc.109.072819 PMC Full Text View journal details
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