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PMID: 1997652 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Thalidomide selectively inhibits tumor necrosis factor alpha production by stimulated human monocytes.

The Journal of experimental medicine ·Vol. 173 ·No. 3 ·1991-03-01 ·Pages 699-703

Sampaio EP, Sarno EN, Galilly R, Cohn ZA, Kaplan G

Abstract

Thalidomide selectively inhibits the production of human monocyte tumor necrosis factor alpha (TNF-alpha) when these cells are triggered with lipopolysaccharide and other agonists in culture. 40% inhibition occurs at the clinically achievable dose of the drug of 1 micrograms/ml. In contrast, the amount of total protein and individual proteins labeled with [35S]methionine and expressed on SDS-PAGE are not influenced. The amounts of interleukin 1 beta (IL-1 beta), IL-6, and granulocyte/macrophage colony-stimulating factor produced by monocytes remain unaltered. The selectivity of this drug may be useful in determining the role of TNF-alpha in vivo and modulating its toxic effects in a clinical setting.

MeSH Terms
Blood Proteins/biosynthesis,isolation & purification Cytokines/biosynthesis,blood Endotoxins/pharmacology Humans In Vitro Techniques Kinetics Lipopolysaccharides/pharmacology Molecular Weight Monocytes/drug effects,physiology Salmonella Thalidomide/pharmacology Tumor Necrosis Factor-alpha/biosynthesis
Chemicals
Blood Proteins Cytokines Endotoxins Lipopolysaccharides Tumor Necrosis Factor-alpha Thalidomide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sampaio E P
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.
Sarno E N
Galilly R
Cohn Z A
Kaplan G
References (20)
20 references, click to expand
  1. Reactions in leprosy.
    Lepr Rev. 1969 Apr;40(2):77-81 PMID: 5792378
  2. The saga of thalidomide. Neuropathy to embryopathy, with case reports of congenital anomalies.
    N Engl J Med. 1962 Dec 6;267:1184-92 contd PMID: 13934699
  3. Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens.
    J Immunol Methods. 1984 Mar 16;67(2):379-88 PMID: 6200537
  4. Thalidomide--effect on T cell subsets as a possible mechanism of action.
    Int J Lepr Other Mycobact Dis. 1985 Jun;53(2):201-5 PMID: 3874920
  5. Direct stimulation of monocyte release of interleukin 1 by mycobacterial protein antigens.
    J Immunol. 1986 Jan;136(1):193-6 PMID: 3484390
  6. Control of cachectin (tumor necrosis factor) synthesis: mechanisms of endotoxin resistance.
    Science. 1986 May 23;232(4753):977-80 PMID: 3754653
  7. Raised serum levels of tumour necrosis factor in parasitic infections.
    Lancet. 1986 Dec 13;2(8520):1364-5 PMID: 2878227
  8. Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9675-8 PMID: 3491987
  9. Tumors secreting human TNF/cachectin induce cachexia in mice.
    Cell. 1987 Aug 14;50(4):555-63 PMID: 3607879
  10. Evidence for tumour necrosis factor/cachectin production in cancer.
    Lancet. 1987 Nov 28;2(8570):1229-32 PMID: 2890853
  11. Cachectin/tumor necrosis factor induces cachexia, anemia, and inflammation.
    J Exp Med. 1988 Mar 1;167(3):1211-27 PMID: 3351436
  12. Tumor necrosis factor and interleukin-1 in the serum of children with severe infectious purpura.
    N Engl J Med. 1988 Aug 18;319(7):397-400 PMID: 3135497
  13. Elevated levels of circulating cachectin/tumor necrosis factor in patients with acquired immunodeficiency syndrome.
    Am J Med. 1988 Sep;85(3):289-91 PMID: 3414726
  14. Acylation of monocyte and glomerular mesangial cell proteins. Myristyl acylation of the interleukin 1 precursors.
    J Clin Invest. 1988 Nov;82(5):1479-88 PMID: 3263392
  15. The complex pattern of cytokines in serum from patients with meningococcal septic shock. Association between interleukin 6, interleukin 1, and fatal outcome.
    J Exp Med. 1989 Jan 1;169(1):333-8 PMID: 2783334
  16. Regulation of the expression of IL-6 in human monocytes.
    J Immunol. 1989 Jun 15;142(12):4339-45 PMID: 2786029
  17. Evidence that tumor necrosis factor has an important role in antibacterial resistance.
    J Immunol. 1989 Nov 1;143(9):2894-9 PMID: 2509559
  18. Induction of interleukin 1 and tumor necrosis factor by mycobacterial proteins: the monocyte western blot.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3348-52 PMID: 2110362
  19. Local production of tumor necrosis factor and IFN-gamma in tuberculous pleuritis.
    J Immunol. 1990 Jul 1;145(1):149-54 PMID: 2113553
  20. Thalidomide: use and possible mode of action in reactional lepromatous leprosy and in various other conditions.
    J Am Acad Dermatol. 1982 Sep;7(3):317-23 PMID: 7130490
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.3.699
Published
1991-03-01
Pages
699-703
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118820
Subset
IM
Grants
NIAID NIH HHS · AI-22616 · United States
External Links
PubMed source DOI: 10.1084/jem.173.3.699 PMC Full Text View journal details
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