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PMID: 19966839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissecting differential gene expression within the circadian neuronal circuit of Drosophila.

Nature neuroscience ·Vol. 13 ·No. 1 ·2010-01-00 ·Pages 60-8

Nagoshi E, Sugino K, Kula E, Okazaki E, Tachibana T, Nelson S, Rosbash M

Abstract

Behavioral circadian rhythms are controlled by a neuronal circuit consisting of diverse neuronal subgroups. To understand the molecular mechanisms underlying the roles of neuronal subgroups within the Drosophila circadian circuit, we used cell-type specific gene-expression profiling and identified a large number of genes specifically expressed in all clock neurons or in two important subgroups. Moreover, we identified and characterized two circadian genes, which are expressed specifically in subsets of clock cells and affect different aspects of rhythms. The transcription factor Fer2 is expressed in ventral lateral neurons; it is required for the specification of lateral neurons and therefore their ability to drive locomotor rhythms. The Drosophila melanogaster homolog of the vertebrate circadian gene nocturnin is expressed in a subset of dorsal neurons and mediates the circadian light response. The approach should also enable the molecular dissection of many different Drosophila neuronal circuits.

MeSH Terms
Animals Animals, Genetically Modified Behavior, Animal Biological Clocks/genetics Brain/cytology Circadian Rhythm/genetics Drosophila Proteins/genetics Drosophila melanogaster Gene Expression Profiling/methods Gene Expression Regulation/physiology Luminescent Proteins/genetics Nerve Net/metabolism Neurons/metabolism Nuclear Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis/methods RNA, Messenger/metabolism Transcription Factors/genetics,metabolism
Chemicals
Drosophila Proteins Luminescent Proteins Nuclear Proteins RNA, Messenger Transcription Factors nocturnin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nagoshi Emi
Howard Hughes Medical Institute, National Center for Behavioral Genomics, Department of Biology, Brandeis University, Waltham, Massachusetts, USA.
Sugino Ken
Kula Ela
Okazaki Etsuko
Tachibana Taro
Nelson Sacha
Rosbash Michael
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Article Info
Journal
Nature neuroscience
Abbr.
Nat Neurosci
ISSN
1546-1726
DOI
10.1038/nn.2451
Published
2010-01-00
Epub
2009-00-06
Pages
60-8
Language
English
Region
United States
NLM ID
9809671
PMCID
PMC3878269
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIMH NIH HHS · R01 MH066338 · United States
Databases
GEO
GSE17803
External Links
PubMed source DOI: 10.1038/nn.2451 PMC Full Text View journal details
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