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PMID: 11394964 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse.

BMC developmental biology ·Vol. 1 ·2001-00-00 ·Pages 9

Wang Y, Osterbur DL, Megaw PL, Tosini G, Fukuhara C, Green CB, Besharse JC

Abstract

Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a novel protein, the nocturnin cDNA had strong sequence similarity with a C-terminal domain of the yeast transcription factor CCR4, and with mouse and human ESTs. Since its original identification others have cloned mouse and human homologues of nocturnin/CCR4, and we have cloned a full-length cDNA from mouse retina, along with partial cDNAs from human, cow and chicken. The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse. cDNA sequence analysis revealed a high degree of conservation among vertebrate nocturnin/CCR4 homologues along with a possible homologue in Drosophila. Northern analysis of mRNA in C3H/He and C57/Bl6 mice revealed that the mNoc gene is expressed in a broad range of tissues, with greatest abundance in liver, kidney and testis. mNoc is also expressed in multiple brain regions including suprachiasmatic nucleus and pineal gland. Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver. The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

MeSH Terms
Amino Acid Sequence Animals Biological Clocks/physiology Cattle Drosophila Proteins/genetics Genes, Intracisternal A-Particle/genetics Humans Male Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred Strains Molecular Sequence Data Nuclear Proteins Organ Specificity/genetics Protein Biosynthesis Proteins/genetics,physiology RNA, Messenger/biosynthesis Rats Sequence Homology, Nucleic Acid Transcription Factors Xenopus Proteins/genetics
Chemicals
Drosophila Proteins Nuclear Proteins Proteins RNA, Messenger Transcription Factors Xenopus Proteins nocturnin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Y
PEL-FREEZ Clinical Systems, LLC, 9099 North Deerbrook Trail, Brown Deer, WI 53223, USA. ywang@pel-freez.com
Osterbur D L
Megaw P L
Tosini G
Fukuhara C
Green C B
Besharse J C
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Article Info
Journal
BMC developmental biology
Abbr.
BMC Dev Biol
ISSN
1471-213X
DOI
10.1186/1471-213x-1-9
Email
ywang@pel-freez.com
Published
2001-00-00
Epub
2001-00-25
Pages
9
Language
English
Region
England
NLM ID
100966973
PMCID
PMC32249
Subset
IM
Grants
NEI NIH HHS · EY02414 · United States
NEI NIH HHS · EY01931 · United States
DS NIH HHS · DS38483 · United States
NEI NIH HHS · R01 EY002414 · United States
NEI NIH HHS · P30 EY001931 · United States
Databases
GENBANK
AF199491, AF199492, AF199493, AF199494, AF199495, AF199497, AF199498
External Links
PubMed source DOI: 10.1186/1471-213x-1-9 PMC Full Text View journal details
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