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PMID: 1992470 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a conserved cell-type-specific protein in nerve terminals coincides with synaptogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 3 ·1991-02-01 ·Pages 785-9

Catsicas S, Larhammar D, Blomqvist A, Sanna PP, Milner RJ, Wilson MC

Abstract

Contact of axons with target territories results in the formation of synapses, specific junctional complexes that may represent a final stage of neuronal maturation. Synaptosomal-associated protein 25 (SNAP-25) is a component of particular nerve terminals recently identified in rodent brain. To evaluate the structure and regulation of molecular components of the synapse, we investigated the expression of SNAP-25 in the developing chicken nervous system. Analysis of SNAP-25 cDNA clones demonstrated that the chicken homologue is identical in amino acid sequence to the mouse protein. In chicken retina and neural tube, the onset of SNAP-25 mRNA and protein expression was found to correspond to the time of synaptogenesis. These results suggest that SNAP-25 plays a role in the physiology of mature nerve terminals and that its expression may be regulated by specific cell-cell interactions occurring during synapse formation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain/embryology,physiology Chick Embryo Chickens Cloning, Molecular Ganglia, Spinal/physiology Gene Library Immunoblotting Immunohistochemistry Membrane Proteins Mice Molecular Sequence Data Nerve Endings/physiology Nerve Tissue Proteins/analysis,genetics Neurons/physiology Nucleic Acid Hybridization RNA, Messenger/genetics Retina/embryology,physiology Sequence Homology, Nucleic Acid Spinal Cord/embryology,physiology Synaptosomal-Associated Protein 25 Synaptosomes/physiology
Chemicals
Membrane Proteins Nerve Tissue Proteins RNA, Messenger Snap25 protein, mouse Synaptosomal-Associated Protein 25
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Catsicas S
Department of Neuropharmacology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Larhammar D
Blomqvist A
Sanna P P
Milner R J
Wilson M C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.3.785
Published
1991-02-01
Pages
785-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50898
Subset
IM
Grants
NINDS NIH HHS · NS22347 · United States
NINDS NIH HHS · NS23038 · United States
Databases
GENBANK
M57957, M67501, M67502, M67503, M67504, M94197, M94198, M94199, M94200, X53420
External Links
PubMed source DOI: 10.1073/pnas.88.3.785 PMC Full Text View journal details
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