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PMID: 1991446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inner membrane protease I, an enzyme mediating intramitochondrial protein sorting in yeast.

The EMBO journal ·Vol. 10 ·No. 2 ·1991-02-00 ·Pages 247-54

Schneider A, Behrens M, Scherer P, Pratje E, Michaelis G, Schatz G

Abstract

Several precursors transported from the cytoplasm to the intermembrane space of yeast mitochondria are first cleaved by the MAS-encoded protease in the matrix space and then by additional proteases that have not been characterized. We have now developed a specific assay for one of these other proteases. The enzyme is an integral protein of the inner membrane; it requires divalent cations and acidic phospholipid for activity, and is defective in yeast mutant pet ts2858 which accumulates an incompletely processed cytochrome b2 precursor. The protease contains a 21.4 kd subunit whose C-terminal part is exposed on the outer face of the inner membrane. An antibody against this polypeptide inhibits the activity of the protease. As overproduction of the polypeptide does not increase the activity of the protease in mitochondria, the enzyme may be a hetero-oligomer. This 'inner membrane protease I' shares several key features with the leader peptidase of Escherichia coli and the signal peptidase of the endoplasmic reticulum.

MeSH Terms
Amino Acid Sequence Antigen-Antibody Complex Doxorubicin/pharmacology Endopeptidases/genetics,isolation & purification,metabolism Immunoglobulin G Intracellular Membranes/enzymology Kinetics Mitochondria/enzymology Mitochondrial Proteins Molecular Sequence Data Mutation Phospholipids/pharmacology Plasmids Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Antigen-Antibody Complex Immunoglobulin G Mitochondrial Proteins Phospholipids Saccharomyces cerevisiae Proteins Doxorubicin Endopeptidases IMP1 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schneider A
Biocenter, University of Basel, Switzerland.
Behrens M
Scherer P
Pratje E
Michaelis G
Schatz G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-02-00
Pages
247-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452640
Subset
IM
Grants
NIGMS NIH HHS · 2R01 GM 37803 · United States
External Links
PubMed source PMC Full Text View journal details
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