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PMID: 19897606 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Histone H2A.Z regulates the expression of several classes of phosphate starvation response genes but not as a transcriptional activator.

Plant physiology ·Vol. 152 ·No. 1 ·2010-01-00 ·Pages 217-25

Smith AP, Jain A, Deal RB, Nagarajan VK, Poling MD, Raghothama KG, Meagher RB

Abstract

Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at these target loci results in their derepression in arp6 mutants and correlates with the presence of multiple Pi-starvation-related phenotypes, including shortened primary roots and increases in the number and length of root hairs, as well as increased starch accumulation and phosphatase activity in shoots. Our data suggest a model for chromatin-level control of Pi starvation responses in which ARP6-dependent H2A.Z deposition modulates the transcription of a suite of PSR genes.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Plant/physiology Histones/genetics,metabolism Illicium Microfilament Proteins/genetics,metabolism Mutation Phosphates Phosphoric Monoester Hydrolases/metabolism Plant Roots/growth & development,metabolism Plant Shoots/growth & development,metabolism Seedlings
Chemicals
ARP6 protein, Arabidopsis Arabidopsis Proteins Histones Microfilament Proteins Phosphates Phosphoric Monoester Hydrolases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Smith Aaron P
Department of Genetics, University of Georgia, Fred C. Davison Life Sciences Complex, Athens, Georgia 30602, USA.
Jain Ajay
Deal Roger B
Nagarajan Vinay K
Poling Michael D
Raghothama Kashchandra G
Meagher Richard B
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.109.145532
Published
2010-01-00
Epub
2009-00-06
Pages
217-25
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2799358
Subset
IM
Grants
NIGMS NIH HHS · GM 36397-22 · United States
External Links
PubMed source DOI: 10.1104/pp.109.145532 PMC Full Text View journal details
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