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PMID: 1987111 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12.

Journal of bacteriology ·Vol. 173 ·No. 1 ·1991-01-00 ·Pages 101-7

Austin D, Larson TJ

Abstract

Aerobic sn-glycerol 3-phosphate dehydrogenase, encoded by the glpD gene of Escherichia coli, is a cytoplasmic membrane-associated respiratory enzyme. The nucleotide sequence of glpD was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a glpD-lacZ translational fusion. The predicted molecular weight, 56,750, corresponds well with the reported value of 58,000 for purified sn-glycerol 3-phosphate dehydrogenase. The flavin-binding domain, located at the amino terminus, was identified by comparison with the amino acid sequences of other flavoproteins from E. coli. Repetitive extragenic palindromic sequences were identified downstream of the glpD coding region. The site for transcription termination was located between 87 and 216 bp downstream of the translation stop codon.

Related Genes
glpD
MeSH Terms
Aerobiosis Amino Acid Sequence Base Sequence Escherichia coli/enzymology,genetics Genes, Bacterial Glycerolphosphate Dehydrogenase/genetics Molecular Sequence Data Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Glycerolphosphate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Austin D
Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Larson T J
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.1.101-107.1991
Published
1991-01-00
Pages
101-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207162
Subset
IM
Databases
GENBANK
J01366, M20938, M55989, S68058, S70324, S70325, S70331, S70358, S70359, S70363, S70365, S70370, X00617
External Links
PubMed source DOI: 10.1128/jb.173.1.101-107.1991 PMC Full Text View journal details
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