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PMID: 1987027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.

Infection and immunity ·Vol. 59 ·No. 1 ·1991-01-00 ·Pages 150-3

Phelps CJ, Lyerly DL, Johnson JL, Wilkins TD

Abstract

Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic, enterotoxic, and lethal. In addition, toxic lysate caused hemagglutination of rabbit erythrocytes. The toxic activities were inhibited by antibody specific for toxin A. Our findings demonstrate that the biological activities exhibited by native toxin A are functions of a single protein encoded by the 8.1-kb toxin A gene, independent of any other C. difficile gene products.

MeSH Terms

Animals Bacterial Toxins Cloning, Molecular Clostridioides difficile/genetics Cricetinae Enterotoxins/genetics,isolation & purification,toxicity Escherichia coli/genetics Gene Expression Genes, Bacterial Recombinant Proteins/isolation & purification

Chemicals

Bacterial Toxins Enterotoxins Recombinant Proteins tcdA protein, Clostridium difficile

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Phelps C J

Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.

Lyerly D L

Johnson J L

Wilkins T D

References (15)

15 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.59.1.150-153.1991

Published

1991-01-00

Pages

150-3

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC257719

Subset

Grants

NIAID NIH HHS · AI 15749 · United States

External Links

PubMed source DOI: 10.1128/iai.59.1.150-153.1991 PMC Full Text View journal details

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Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.

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