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PMID: 19864465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel mouse HSF3 has the potential to activate nonclassical heat-shock genes during heat shock.

Molecular biology of the cell ·Vol. 21 ·No. 1 ·2010-01-01 ·Pages 106-16

Fujimoto M, Hayashida N, Katoh T, Oshima K, Shinkawa T, Prakasam R, Tan K, Inouye S, Takii R, Nakai A

Abstract

The heat-shock response is characterized by the expression of a set of classical heat-shock genes, and is regulated by heat-shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat shock. It is believed that HSF1 is required for the heat-inducible expression of these genes although HSF2 and HSF4 modulate some of the gene expression. Here, we identified a novel mouse HSF3 (mHSF3) translocated into the nucleus during heat shock. However, mHSF3 did not activate classical heat-shock genes such as Hsp70. Remarkably, overexpression of mHSF3 restored the expression of nonclassical heat-shock genes such as PDZK3 and PROM2 in HSF1-null mouse embryonic fibroblasts (MEFs). Although down-regulation of mHSF3 expression had no effect on gene expression or cell survival in wild-type MEF cells, it abolished the moderate expression of PDZK3 mRNA and reduced cell survival in HSF1-null MEF cells during heat shock. We propose that mHSF3 represents a unique HSF that has the potential to activate only nonclassical heat-shock genes to protect cells from detrimental stresses.

MeSH Terms
Animals COS Cells Cell Nucleus/metabolism Chickens Chlorocebus aethiops Cytoprotection DNA/metabolism DNA Helicases/metabolism Gene Expression Regulation Genome/genetics HSP70 Heat-Shock Proteins/genetics,metabolism Heat-Shock Proteins/metabolism Heat-Shock Response/genetics Humans Mice Molecular Sequence Data Multigene Family/genetics Nuclear Proteins/metabolism Protein Binding Protein Transport Stress, Physiological Temperature Transcription Factors/metabolism
Chemicals
HSF3 protein, mouse HSP70 Heat-Shock Proteins Heat-Shock Proteins Nuclear Proteins Transcription Factors DNA Smarca4 protein, mouse DNA Helicases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Fujimoto Mitsuaki
Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Hayashida Naoki
Katoh Takuma
Oshima Kouji
Shinkawa Toyohide
Prakasam Ramachandran
Tan Ke
Inouye Sachiye
Takii Ryosuke
Nakai Akira
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e09-07-0639
Published
2010-01-01
Epub
2009-00-28
Pages
106-16
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2801703
Subset
IM
Databases
GENBANK
AB510446, AB510447, AB510448
External Links
PubMed source DOI: 10.1091/mbc.e09-07-0639 PMC Full Text View journal details
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